| Proteomics of the autographa californica nucleopolyhedrovirus budded virions | |
| Wang, RanRan1,2; Deng, Fei1,2; Hou, Dianhai1,2; Zhao, Yong3,4; Guo, Lin3,4; Wang, Hualin1,2; Hu, Zhihong1,2 | |
| 刊名 | Journal of virology
 |
| 2010-07-01 | |
| 卷号 | 84期号:14页码:7233-7242 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00040-10 |
| 通讯作者 | Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | Baculoviruses produce two progeny phenotypes during their replication cycles. the occlusion-derived virus (odv) is responsible for initiating primary infection in the larval midgut, and the budded virus (bv) phenotype is responsible for the secondary infection. the proteomics of several baculovirus odvs have been revealed, but so far, no extensive analysis of bv-associated proteins has been conducted. in this study, the protein composition of the bv of autographa californica nucleopolyhedrovirus (acmnpv), the type species of baculoviruses, was analyzed by various mass spectrometry (ms) techniques, including liquid chromatography-triple quadrupole linear ion trap (lc-qtrap), liquid chromatography-quadrupole time of flight (lc-q-tof), and matrix-assisted laser desorption ionization-time of flight (maldi-tof). sds-page and maldi-tof analyses showed that the three most abundant proteins of the acmnpv bv were gp64, vp39, and p6.9. a total of 34 viral proteins associated with the acmnpv bv were identified by the indicated methods. thirteen of these proteins, pp31, ac58/59, ac66, iap-2, ac73, ac74, ac114, ac124, chitinase, polyhedron envelope protein (pep), ac132, odv-e18, and odv-e56, were identified for the first time to be bv-associated proteins. western blot analyses showed that odv-e18 and odv-e25, which were previously thought to be odv-specific proteins, were also present in the envelop fraction of bv. in addition, 11 cellular proteins were found to be associated with the acmnpv bv by both lc-qtrap and lc-q-tof analyses. interestingly, seven of these proteins were also identified in other enveloped viruses, suggesting that many enveloped viruses may commonly utilize certain conserved cellular pathways. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS ; OCCLUSION-DERIVED VIRUS ; LITURA MULTICAPSID NUCLEOPOLYHEDROVIRUS ; SINGLE-NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; BACULOVIRUS STRUCTURAL PROTEIN ; ENVELOPE FUSION PROTEIN ; OPEN READING FRAME ; HELICOVERPA-ARMIGERA NUCLEOPOLYHEDROVIRUS ; ORGYIA-PSEUDOTSUGATA BACULOVIRUS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000278935700033 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375788 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China 4.Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, RanRan,Deng, Fei,Hou, Dianhai,et al. Proteomics of the autographa californica nucleopolyhedrovirus budded virions[J]. Journal of virology,2010,84(14):7233-7242. |
| APA | Wang, RanRan.,Deng, Fei.,Hou, Dianhai.,Zhao, Yong.,Guo, Lin.,...&Hu, Zhihong.(2010).Proteomics of the autographa californica nucleopolyhedrovirus budded virions.Journal of virology,84(14),7233-7242. |
| MLA | Wang, RanRan,et al."Proteomics of the autographa californica nucleopolyhedrovirus budded virions".Journal of virology 84.14(2010):7233-7242. |
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