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	Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol
	Liu, Yan; Wei, Qing; Wang, Shu-Jun; Liu, Hong; Zhou, Ning-Yi
刊名	Applied microbiology and biotechnology
	2010-06-01
卷号	87期号:1页码:281-287
关键词	Non-antibiotic resistance gene marker Methyl parathion Ortho-nitrophenol Para-nitrophenol Simultaneous degradation
ISSN号	0175-7598
DOI	10.1007/s00253-010-2457-y
通讯作者	Liu, hong(liuhong@wh.iov.cn)
英文摘要	Pseudomonas sp. strain nyz402, a native soil organism that grows on para-nitrophenol (pnp), was genetically engineered for the simultaneous degradation of methyl parathion (mp) and ortho-nitrophenol (onp) by integrating mph (methyl parathion hydrolase gene) from pseudomonas sp. strain wbc-3 and onpab (onp 2-monooxygenase and onp o-benzoquinone reductase genes) from alcaligenes sp. strain nyz215 into the genome of strain nyz402. methyl parathion hydrolase (mph), onp 2-monooxygenase (onpa) and o-benzoquinone reductase (onpb) were constitutively expressed in the engineered strain nyz-mo. strain nyz-mo was free of exogenous antibiotic resistance gene markers and the introduced genes were genetically stable. degradation experiments showed that strain nyz-mo could utilize mp or onp as the sole carbon and energy source, and mineralize 0.1 mm mp-0.1 mm onp simultaneously. this method may serve as a useful strategy for the construction of engineered strains in the degradation of multiple environmental pollutants.
WOS关键词	GRAM-NEGATIVE BACTERIA ; P-NITROPHENOL ; PSEUDOMONAS-AERUGINOSA ; MOLECULAR CHARACTERIZATION ; CLONING ; DEGRADATION ; CATABOLISM ; CLUSTER ; SYSTEM ; ORGANOPHOSPHATE
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000277784100029
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375780
专题	武汉病毒研究所
通讯作者	Liu, Hong
作者单位	Chinese Acad Sci, State key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yan,Wei, Qing,Wang, Shu-Jun,et al. Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol[J]. Applied microbiology and biotechnology,2010,87(1):281-287.
APA	Liu, Yan,Wei, Qing,Wang, Shu-Jun,Liu, Hong,&Zhou, Ning-Yi.(2010).Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol.Applied microbiology and biotechnology,87(1),281-287.
MLA	Liu, Yan,et al."Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol".Applied microbiology and biotechnology 87.1(2010):281-287.

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