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	Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice
	Lu, Baojing1,2; Huang, Yi1; Huang, Li1; Li, Bao2; Zheng, Zhenhua1; Chen, Ze1; Chen, Jianjun1; Hu, Qinxue1; Wang, Hanzhong1
刊名	Immunology
	2010-06-01
卷号	130期号:2页码:254-261
关键词	Cytosine-phosphate-guanosine (cpg) Mucosal immunization Severe acute respiratory syndrome coronavirus (sars-cov) Virus-like particles
ISSN号	0019-2805
DOI	10.1111/j.1365-2567.2010.03231.x
通讯作者	Wang, hanzhong(wanghz@wh.iov.cn)
英文摘要	P>nasal administration has emerged as a promising and attractive route for vaccination, especially for the prophylaxis of respiratory diseases. our previous studies have shown that severe acute respiratory syndrome coronavirus (sars-cov) virus-like particles (vlps) can be assembled using a recombinant baculovirus (rbv) expression system and such vlps induce specific humoral and cellular immune responses in mice after subcutaneous injection. here, we investigated mucosal immune responses to sars-cov vlps in a mouse model. mice were immunized in parallel, intraperitoneally or intranasally, with vlps alone or with vlps plus cytosine-phosphate-guanosine (cpg). immune responses, including the production of sars-cov-specific serum immunoglobulin g (igg) and secretory immunoglobulin a (siga), were determined in mucosal secretions and tissues. both immunizations induced sars-cov-specific igg, although the levels of igg in groups immunized via the intraperitoneal (i.p.) route were higher. siga was detected in saliva in groups immunized intranasally but not in groups immunized intraperitoneally. cpg had an adjuvant effect on iga production in genital tract washes when administered intranasally but only affected iga production in faeces samples when administered intraperitoneally. in addition, iga was also detected in mucosal tissues from the lung and intestine, while cpg induced an increased level of iga in the intestine. most importantly, neutralization antibodies were detected in sera after i.p. and intranasal (i.n.) immunizations. secretions in genital tract washes from the i.n. group also showed neutralization activity. furthermore, vlps that were administered intraperitoneally elicited cellular immune responses as demonstrated by enzyme-linked immunospot (elispot) assay analyses. in summary, our study indicates that mucosal immunization with rbv sars-cov vlps represent an effective means for eliciting protective systemic and mucosal immune responses against sars-cov, providing important information for vaccine design.
WOS关键词	B SURFACE-ANTIGEN ; IMMUNE-RESPONSES ; CPG DNA ; SPIKE GLYCOPROTEIN ; POTENT ENHANCER ; INSECT CELLS ; VACCINE ; ADJUVANT ; IMMUNOGENICITY ; IDENTIFICATION
WOS研究方向	Immunology
WOS类目	Immunology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000277411900011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375756
专题	武汉病毒研究所
通讯作者	Wang, Hanzhong
作者单位	1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Anhui Med Univ, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Lu, Baojing,Huang, Yi,Huang, Li,et al. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice[J]. Immunology,2010,130(2):254-261.
APA	Lu, Baojing.,Huang, Yi.,Huang, Li.,Li, Bao.,Zheng, Zhenhua.,...&Wang, Hanzhong.(2010).Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice.Immunology,130(2),254-261.
MLA	Lu, Baojing,et al."Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice".Immunology 130.2(2010):254-261.

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