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	A single dose of dna vaccine based on conserved h5n1 subtype proteins provides protection against lethal h5n1 challenge in mice pre-exposed to h1n1 influenza virus
	Chang, Haiyan2; Huang, Chaoyang2; Wu, Jian2; Fang, Fang2; Zhang, Wenjie5; Wang, Fuyan1; Chen, Ze2,3,4
刊名	Virology journal
	2010-08-21
卷号	7页码:9
ISSN号	1743-422X
DOI	10.1186/1743-422x-7-197
通讯作者	Wang, fuyan(wfy4010@163.com)
英文摘要	Background: highly pathogenic avian influenza virus subtype h5n1 infects humans with a high fatality rate and has pandemic potential. vaccination is the preferred approach for prevention of h5n1 infection. seasonal influenza virus infection has been reported to provide heterosubtypic immunity against influenza a virus infection to some extend. in this study, we used a mouse model pre-exposed to an h1n1 influenza virus and evaluated the protective ability provided by a single dose of dna vaccines encoding conserved h5n1 proteins. results: spf balb/c mice were intranasally infected with a/pr8 (h1n1) virus beforehand. six weeks later, the mice were immunized with plasmid dna expressing h5n1 virus np or m1, or with combination of the two plasmids. both serum specific ab titers and ifn-gamma secretion by spleen cells in vitro were determined. six weeks after the vaccination, the mice were challenged with a lethal dose of h5n1 influenza virus. the protective efficacy was judged by survival rate, body weight loss and residue virus titer in lungs after the challenge. the results showed that pre-exposure to h1n1 virus could offer mice partial protection against lethal h5n1 challenge and that single-dose injection with np dna or np + m1 dnas provided significantly improved protection against lethal h5n1 challenge in mice pre-exposed to h1n1 virus, as compared with those in unexposed mice. conclusions: pre-existing immunity against seasonal influenza viruses is useful in offering protection against h5n1 infection. dna vaccination may be a quick and effective strategy for persons innaive to influenza a virus during h5n1 pandemic.
WOS关键词	EXPRESSING PLASMID DNAS ; A H5N1 ; ANTIBODY-RESPONSES ; CROSS-PROTECTION ; SAFETY ; IMMUNOGENICITY ; INFECTION ; HEMAGGLUTININ ; NEURAMINIDASE ; NUCLEOPROTEIN
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000282604300001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375755
专题	武汉病毒研究所
通讯作者	Wang, Fuyan
作者单位	1.Cent S Univ, Xiangya Sch Med, Dept Immunol, Changsha 410078, Hunan, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 3.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 5.Shanghai Jiao Tong Univ, Xinhua Hosp, Shanghai 200092, Peoples R China
推荐引用方式 GB/T 7714	Chang, Haiyan,Huang, Chaoyang,Wu, Jian,et al. A single dose of dna vaccine based on conserved h5n1 subtype proteins provides protection against lethal h5n1 challenge in mice pre-exposed to h1n1 influenza virus[J]. Virology journal,2010,7:9.
APA	Chang, Haiyan.,Huang, Chaoyang.,Wu, Jian.,Fang, Fang.,Zhang, Wenjie.,...&Chen, Ze.(2010).A single dose of dna vaccine based on conserved h5n1 subtype proteins provides protection against lethal h5n1 challenge in mice pre-exposed to h1n1 influenza virus.Virology journal,7,9.
MLA	Chang, Haiyan,et al."A single dose of dna vaccine based on conserved h5n1 subtype proteins provides protection against lethal h5n1 challenge in mice pre-exposed to h1n1 influenza virus".Virology journal 7(2010):9.

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