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	Hepatitis b virus/hepatitis c virus upregulate angiopoietin-2 expression through mitogen-activated protein kinase pathway
	Li, Yanmei1; Chen, Jizheng1; Wu, Chunchen1; Wang, Linding1; Lu, Mengji1,2; Chen, Xinwen1
刊名	Hepatology research
	2010-10-01
卷号	40期号:10页码:1022-1033
关键词	Angiopoietin-2 Ap-1 Ets1 Hepatitis b virus Hepatitis c virus Hepatocellular carcinoma Mitogen-activated protein kinase
ISSN号	1386-6346
DOI	10.1111/j.1872-034x.2010.00712.x
通讯作者	Chen, xinwen(chenxw@pentium.whiov.ac.cn)
英文摘要	Aim: to explore the molecular mechanism of hepatitis b virus (hbv)/hepatitis c virus (hcv) upregulate angiopoietin-2 (ang-2) expression. methods: reverse transcription polymerase chain reaction (rt-pcr), quantitative real-time (qrt)-pcr and enzyme-linked immunosorbent assay (elisa) analysis were used to measure the ang-2 transcription and expression level. reporter gene assays were used to determine the cis-element of the ang-2 promoter. the specific inhibitors assay, immunofluorescence and western blot analysis were conducted to verify the signal pathway involved in the upregulation of ang-2 expression. results: the level of transcription and expression of ang-2 increased in the hepg2.2.15 and con-1 cells. reporter gene assays in hepg2.2.15 and con-1 cells revealed that hbv/hcv could enhance ang-2 promoter expression by activating ap-1 and ets1. analysis with specific inhibitors indicated that hbv/hcv upregulated the expression of ang-2 through mitogen-activated protein kinase (mapk) pathways. conclusion: this study illustrates a distinct mechanism by which a tumor virus modulates vasculature to promote tumorigenesis.
WOS关键词	ENDOTHELIAL GROWTH-FACTOR ; HUMAN HEPATOCELLULAR-CARCINOMA ; NONPARENCHYMAL LIVER-CELLS ; VIRAL REPLICATION ; ANGIOGENESIS ; SURVIVAL ; HEPATOCARCINOGENESIS ; TRANSCRIPTION ; ETS-1 ; VEGF
WOS研究方向	Gastroenterology & Hepatology
WOS类目	Gastroenterology & Hepatology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000282372000011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375751
专题	武汉病毒研究所
通讯作者	Chen, Xinwen
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Hosp, Inst Virol, Essen, Germany
推荐引用方式 GB/T 7714	Li, Yanmei,Chen, Jizheng,Wu, Chunchen,et al. Hepatitis b virus/hepatitis c virus upregulate angiopoietin-2 expression through mitogen-activated protein kinase pathway[J]. Hepatology research,2010,40(10):1022-1033.
APA	Li, Yanmei,Chen, Jizheng,Wu, Chunchen,Wang, Linding,Lu, Mengji,&Chen, Xinwen.(2010).Hepatitis b virus/hepatitis c virus upregulate angiopoietin-2 expression through mitogen-activated protein kinase pathway.Hepatology research,40(10),1022-1033.
MLA	Li, Yanmei,et al."Hepatitis b virus/hepatitis c virus upregulate angiopoietin-2 expression through mitogen-activated protein kinase pathway".Hepatology research 40.10(2010):1022-1033.

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