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	Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus
	Peng, Ke; Wu, Minzhi; Deng, Fei; Song, Jingjiao; Dong, Chunsheng; Wang, Hualin; Hu, Zhihong1
刊名	Journal of general virology
	2010-03-01
卷号	91页码:659-670
ISSN号	0022-1317
DOI	10.1099/vir.0.017103-0
通讯作者	Hu, zhihong(huzh@wh.iov.cn)
英文摘要	The purpose of this study was to identify protein-protein interactions among the components of the occlusion-derived virus (odv) of helicoverpa armigera nucleopolyhedrovirus (hearnpv), a group 11 alphabaculovirus in the family baculoviridae. to achieve this, 39 selected genes of potential odv structural proteins were cloned and expressed in the gal4 yeast two-hybrid (y2h) system. the direct-cross y2h assays identified 22 interactions comprising 13 binary interactions [ha9-odv-ec43, odv-e56-38k, odv-e56-pif3, lef3-helicase, lef3-alkaline nuclease (an), gp41-38k, gp41-ha90, 38k-pif3,38k-pif2, vp80-ha100, odv-e66-pif3, odv-e66-pif2 and pif3-pif2] and nine self-associations (ie1, ha44, lef3, ha66, gp41, cg30, 38k, pif3 and p24). five of these interactions - lef3-helicase and lef3-an, and the self-associations of ie1, lef3 and 38k - have been reported previously in autographa californica multiple nucleopolyhedrovirus. as ha44 and ha100 were two newly identified odv proteins of group 11 viruses, their interactions were further confirmed. the self-association of ha44 was verified with a his pull-down assay and the interaction of vp80-ha100 was confirmed by a coimmunoprecipitation assay. a summary of the protein-protein interactions of baculoviruses reported so far, comprising 68 interactions with 45 viral proteins and five host proteins, is presented, which will facilitate our understanding of the molecular mechanisms of baculovirus infection.
WOS关键词	CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS ; SINGLE NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS ; NUCLEAR POLYHEDROSIS-VIRUS ; DNA-BINDING-PROTEIN ; BACULOVIRUS TRANSREGULATOR IE1 ; HELIOTHIS-VIRESCENS LARVAE ; ACMNPV EXONO AC141 ; STRUCTURAL PROTEIN ; MULTICAPSID NUCLEOPOLYHEDROVIRUS
WOS研究方向	Biotechnology & Applied Microbiology ; Virology
WOS类目	Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000275587200009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375738
专题	武汉病毒研究所
通讯作者	Hu, Zhihong
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Peng, Ke,Wu, Minzhi,Deng, Fei,et al. Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus[J]. Journal of general virology,2010,91:659-670.
APA	Peng, Ke.,Wu, Minzhi.,Deng, Fei.,Song, Jingjiao.,Dong, Chunsheng.,...&Hu, Zhihong.(2010).Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus.Journal of general virology,91,659-670.
MLA	Peng, Ke,et al."Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus".Journal of general virology 91(2010):659-670.

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