| Molecular anatomy of subcellular localization of hsv-1 tegument protein us11 in living cells | |
| Xing, Junji; Wu, Fuqing; Pan, Weiwei; Zheng, Chunfu | |
| 刊名 | Virus research
 |
| 2010-10-01 | |
| 卷号 | 153期号:1页码:71-81 |
| 关键词 | Herpes simplex virus 1 us11 protein Nucleolar localization signal Nuclear export signal Ran-gtp Importin |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2010.07.009 |
| 通讯作者 | Zheng, chunfu(zheng.alan@hotmail.com) |
| 英文摘要 | The herpes simplex virus type i (hsv-1) us11 protein is an rna-binding multifunctional regulator that specifically and stably associates with nucleoli. although the c-terminal part of us11 was responsible for its nucleolar localization, the precise nucleolar localization signal (nols) and nuclear export signal (nes) of us11 and its nuclear import and export mechanisms are still elusive. in this study, fluorescence microscopy was employed to investigate the subcellular localization of us11 and characterize its transport mechanism in living cells. by constructing a series of deletion mutants fused with enhanced yellow fluorescent protein (eyfp), three novel nolss of us11 were for the first time mapped to amino acids 84-125, 126-152, and 89-146, respectively. additionally, the nes was identified to locate between amino acids 89 and 119. furthermore, the us11 protein was demonstrated to target to the cytoplasm through the nes by chromosomal region maintenance 1 (crm1)-independent pathway, and to the nucleolus through ran and importin beta-dependent mechanism that does not require importin alpha 5. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | SIMPLEX-VIRUS TYPE-1 ; RNA-BINDING PROTEIN ; NUCLEAR EXPORT SIGNALS ; LARGE T-ANTIGEN ; INFECTED-CELLS ; NUCLEOLAR LOCALIZATION ; GENE-PRODUCT ; POSTTRANSCRIPTIONAL TRANSACTIVATION ; NUCLEOCYTOPLASMIC TRANSPORT ; MESSENGER-RNA |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000282486100010 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375737 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Chunfu |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Mol Virol & Viral Immunol Res Grp, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing, Junji,Wu, Fuqing,Pan, Weiwei,et al. Molecular anatomy of subcellular localization of hsv-1 tegument protein us11 in living cells[J]. Virus research,2010,153(1):71-81. |
| APA | Xing, Junji,Wu, Fuqing,Pan, Weiwei,&Zheng, Chunfu.(2010).Molecular anatomy of subcellular localization of hsv-1 tegument protein us11 in living cells.Virus research,153(1),71-81. |
| MLA | Xing, Junji,et al."Molecular anatomy of subcellular localization of hsv-1 tegument protein us11 in living cells".Virus research 153.1(2010):71-81. |
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