QQ客服 官方微博 反馈留言

	Human cellular protein nucleoporin hnup98 interacts with influenza a virus ns2/nuclear export protein and overexpression of its glfg repeat domain can inhibit virus propagation
	Chen, Jingjing1,2; Huang, Shengping1,2; Chen, Ze1,3,4
刊名	Journal of general virology
	2010-10-01
卷号	91页码:2474-2484
ISSN号	0022-1317
DOI	10.1099/vir.0.022681-0
通讯作者	Chen, ze()
英文摘要	The non-structural protein ns2, also called nuclear export protein, of influenza a virus contains a leucine-rich nuclear-export signal that could guide viral ribonucleoproteins to cross the nuclear pore complex (npc) and complete directional nucleocytoplasmic trafficking. in this study, human nucleoporin 98 (hnup98), an npc protein, was identified as an ns2-binding protein by using yeast two-hybrid screening of a human cdna library interaction between ns2 and hnup98 was confirmed in yeast and mammalian cells. mapping tests further demonstrated that aa 22-53 in the n-terminal region of ns2 and the glycine-leucine-phenylalanine-glycine (glfg) repeat domain (aa 1-511) of hnup98 are crucial for the interaction of these two proteins confocal microscopy showed that hnup98 could specifically recruit ns2 to the nucleoli and that this process was inhibited by leptomycin b, a specific inhibitor of human chromosomal region maintenance 1 protein. ns2 recruitment to the nucleoli was through the n-terminal glfg repeat domain of hnup98, but not through the c-terminal domain moreover, influenza virus infection downregulated nup98 levels significantly in 293t and madin-darby canine kidney cells overexpression of the glfg repeat domain of hnup98 apparently inhibited virus propagation together, these findings reveal the interaction between hnup98 and ns2 the glfg repeat domain of hnup98 might competitively inhibit the interaction between ns2 and endogenous hnup98, consequently inhibiting virus propagation
WOS关键词	NUCLEAR-PORE COMPLEX ; MESSENGER-RNA EXPORT ; NS2 PROTEIN ; TYPE-1 REV ; IMPORTIN-BETA ; NUP98 ; TRAFFICKING ; TRANSPORT ; NEP ; REPLICATION
WOS研究方向	Biotechnology & Applied Microbiology ; Virology
WOS类目	Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000283004800008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375701
专题	武汉病毒研究所
通讯作者	Chen, Ze
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 4.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jingjing,Huang, Shengping,Chen, Ze. Human cellular protein nucleoporin hnup98 interacts with influenza a virus ns2/nuclear export protein and overexpression of its glfg repeat domain can inhibit virus propagation[J]. Journal of general virology,2010,91:2474-2484.
APA	Chen, Jingjing,Huang, Shengping,&Chen, Ze.(2010).Human cellular protein nucleoporin hnup98 interacts with influenza a virus ns2/nuclear export protein and overexpression of its glfg repeat domain can inhibit virus propagation.Journal of general virology,91,2474-2484.
MLA	Chen, Jingjing,et al."Human cellular protein nucleoporin hnup98 interacts with influenza a virus ns2/nuclear export protein and overexpression of its glfg repeat domain can inhibit virus propagation".Journal of general virology 91(2010):2474-2484.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。