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	Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci
	Cui, Zongqiang1; Zhang, Ke1,2; Zhang, Zhiping1; Liu, Yalan1,2; Zhou, Yafeng1; Wei, Hongping1; Zhang, Xian-En1
刊名	Virology
	2009-09-30
卷号	392期号:2页码:169-177
关键词	Hcmv pp65 Dynamic accumulation Nuclear foci Self-interact Frap Fret
ISSN号	0042-6822
DOI	10.1016/j.virol.2009.06.021
通讯作者	Zhang, xian-en(x.zhang@wh.iov.cn)
英文摘要	The phosphorylated protein pp65 of human cytomegalovirus (hcmv) is the predominant virion protein and the major tegument constituent. it plays important roles in hcmv infection and virion assembly. live cell imaging and fluorescence recovery after photobleaching (frap) analysis showed that hcmv pp65 accumulated dynamically ill punctuate nuclear foci when transiently expressed in mammalian cells. fluorescence resonance energy transfer (fret) imaging disclosed that pp65 can self-interact in its localization foci. yeast two-hybrid assay verified that pp65 is a self-associating protein, and the n-terminal amino acids 14-22 were determined to be essential for pp65 self-association. however, these amino acids were not related to pp65 localization in the specific nuclear foci. the interaction of pp65 and ppul97 was also studied by fret microscopy, and the result suggested that there is another signal sequence in pp65, being the ppul97 phosphorylation site, that is responsible for localization of pp65 in nuclear foci. these results help to understand the function of pp65 in hcmv infection and virion morphogenesis. (c) 2009 elsevier inc. all rights reserved.
WOS关键词	TEGUMENT PROTEIN PP65 ; RESONANCE ENERGY-TRANSFER ; STRUCTURAL PROTEINS ; LOCALIZATION ; KINASE ; MATRIX ; VIRION ; REPLICATION ; UL83 ; FIBROBLASTS
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000270247300003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375598
专题	武汉病毒研究所
通讯作者	Zhang, Xian-En
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Cui, Zongqiang,Zhang, Ke,Zhang, Zhiping,et al. Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci[J]. Virology,2009,392(2):169-177.
APA	Cui, Zongqiang.,Zhang, Ke.,Zhang, Zhiping.,Liu, Yalan.,Zhou, Yafeng.,...&Zhang, Xian-En.(2009).Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci.Virology,392(2),169-177.
MLA	Cui, Zongqiang,et al."Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci".Virology 392.2(2009):169-177.

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