| Detection of b. anthracis spores and vegetative cells with the same monoclonal antibodies | |
| Wang, Dian-Bing; Yang, Ruifu; Zhang, Zhi-Ping; Bi, Li-Jun; You, Xiang-Yu; Wei, Hong-Ping; Zhou, Ya-Feng; Yu, Ziniu; Zhang, Xian-En | |
| 刊名 | Plos one
 |
| 2009-11-13 | |
| 卷号 | 4期号:11页码:9 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0007810 |
| 通讯作者 | Wang, dian-bing() |
| 英文摘要 | Bacillus anthracis, the causative agent of anthrax disease, could be used as a biothreat reagent. it is vital to develop a rapid, convenient method to detect b. anthracis. in the current study, three high affinity and specificity monoclonal antibodies (mabs, designated 8g3, 10c6 and 12f6) have been obtained using fully washed b. anthracis spores as an immunogen. these mabs, confirmed to direct against ea1 protein, can recognize the surface of b. anthracis spores and intact vegetative cells with high affinity and species-specificity. ea1 has been well known as a major s-layer component of b. anthracis vegetative cells, and it also persistently exists in the spore preparations and bind tightly to the spore surfaces even after rigorous washing. therefore, these mabs can be used to build a new and rapid immunoassay for detection of both life forms of b. anthracis, either vegetative cells or spores. |
| WOS关键词 | BACILLUS-CEREUS GROUP ; GENETIC-RELATIONSHIPS ; MASS-SPECTROMETRY ; S-LAYER ; PCR ; SUBTILIS ; THURINGIENSIS ; COMPONENT ; BACTERIA ; SEQUENCE |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000271721900004 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375591 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Dian-Bing |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dian-Bing,Yang, Ruifu,Zhang, Zhi-Ping,et al. Detection of b. anthracis spores and vegetative cells with the same monoclonal antibodies[J]. Plos one,2009,4(11):9. |
| APA | Wang, Dian-Bing.,Yang, Ruifu.,Zhang, Zhi-Ping.,Bi, Li-Jun.,You, Xiang-Yu.,...&Zhang, Xian-En.(2009).Detection of b. anthracis spores and vegetative cells with the same monoclonal antibodies.Plos one,4(11),9. |
| MLA | Wang, Dian-Bing,et al."Detection of b. anthracis spores and vegetative cells with the same monoclonal antibodies".Plos one 4.11(2009):9. |
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