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	Species-specific cell wall binding affinity of the s-layer proteins of mosquitocidal bacterium bacillus sphaericus c3-41
	Li, Jia1,2; Hu, Xiaomin1; Yan, Jianpin1; Yuan, Zhiming1
刊名	Applied and environmental microbiology
	2009-06-15
卷号	75期号:12页码:3891-3895
ISSN号	0099-2240
DOI	10.1128/aem.00356-09
通讯作者	Yuan, zhiming(yzm@wh.iov.cn)
英文摘要	The binding affinities and specificities of six truncated s-layer homology domain (slh) polypeptides of mosquitocidal bacillus sphaericus strain c3-41 with the purified cell wall sacculi have been assayed. the results indicated that the slh polypeptide comprised of amino acids 31 to 210 was responsible for anchoring the s-layer subunits to the rigid cell wall layer via a distinct type of secondary cell wall polymer and that a motif of the recombinant slh polypeptide comprising amino acids 152 to 210 (rslh(152-210)) was essential for the stable binding of the s-layer with the bacterial cell walls. the quantitative assays revealed that the k(d) (equilibrium dissociation constant) values of rslh(152-210) and rslh(31-210) with purified cell wall sacculi were 1.11 x 10(-6) m and 1.40 x 10(-6) m, respectively. the qualitative assays demonstrated that the slh domain of strain c3-41 could bind only to the cell walls or the cells treated with 5 m guanidinium hydrochloride of both toxic and nontoxic b. sphaericus strains but not to those from other bacteria, indicating the species-specific binding of the slh polypeptide of b. sphaericus with bacterial cell walls.
WOS关键词	CRYSTALLINE SURFACE-LAYERS ; CLOSTRIDIUM-THERMOCELLUM ; HOMOLOGY DOMAINS ; SLH DOMAIN ; POLYMER ; IDENTIFICATION ; PEPTIDOGLYCAN ; SEQUENCE ; STRAINS ; REGION
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000266782300008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375571
专题	武汉病毒研究所
通讯作者	Yuan, Zhiming
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Jia,Hu, Xiaomin,Yan, Jianpin,et al. Species-specific cell wall binding affinity of the s-layer proteins of mosquitocidal bacterium bacillus sphaericus c3-41[J]. Applied and environmental microbiology,2009,75(12):3891-3895.
APA	Li, Jia,Hu, Xiaomin,Yan, Jianpin,&Yuan, Zhiming.(2009).Species-specific cell wall binding affinity of the s-layer proteins of mosquitocidal bacterium bacillus sphaericus c3-41.Applied and environmental microbiology,75(12),3891-3895.
MLA	Li, Jia,et al."Species-specific cell wall binding affinity of the s-layer proteins of mosquitocidal bacterium bacillus sphaericus c3-41".Applied and environmental microbiology 75.12(2009):3891-3895.

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