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	Functional characterization of flgm in the regulation of flagellar synthesis and motility in yersinia pseudotuberculosis
	Ding, Lisha1,3; Wang, Yao1; Hu, Yangbo1,3; Atkinson, Steve2; Williams, Paul2; Chen, Shiyun1
刊名	Microbiology-sgm
	2009-06-01
卷号	155页码:1890-1900
ISSN号	1350-0872
DOI	10.1099/mic.0.026294-0
通讯作者	Chen, shiyun(sychen@wh.iov.cn)
英文摘要	We describe here the functional characterization of the flgm gene in yersinia pseudotuberculosis. direct interaction of flgm with the alternative sigma factor sigma(28) (flia) was first confirmed. a conserved region in the c-terminus of flgm was found which included the sigma(28) binding domain. by site-directed mutagenesis, bacterial two-hybrid analysis and western blotting, the primary flgm binding sites with sigma(28) were shown to be ile85, ala86 and leu89. a role for flgm in swimming motility was demonstrated by inactivation of flgm and subsequent complementation in trans. transcriptional fusion analyses showed differential gene expression of flhdc, flia, flgm and mc in the flia and flgm mutants compared with the wild-type. flhdc expression was not influenced by sigma(28) or flgm while ma expression was abolished in the flia mutant and considerably reduced in the flgm mutant when compared to the wild-type, indicating that both flia and flgm can activate flia transcription. conversely, flgm transcription was higher in the flia mutant when compared to the wild-type, suggesting that flgm transcription was repressed by sigma(28). interestingly, flic expression was markedly increased in the flgm mutant, suggesting a negative regulatory role for flgm in flic expression. the transcription of other sigma-dependent genes (chew, flgd, flaa, csra and fliz) was also examined in flia and flgm mutant backgrounds and this revealed that other sigma-factors apart from sigma(28) may be involved in flagellar biogenesis in y. pseudotuberculosis. taking together the motility phenotypes and effects of flgm mutation on the regulation of these key motility genes, we propose that the mechanisms regulating flagellar biogenesis in y. pseudo tuberculosis may differ from those described for other bacteria.
WOS关键词	ANTI-SIGMA FACTOR ; ENTERICA SEROVAR TYPHIMURIUM ; SALMONELLA-TYPHIMURIUM ; ESCHERICHIA-COLI ; HELICOBACTER-PYLORI ; VIBRIO-CHOLERAE ; TRANSCRIPTIONAL REGULATION ; ANTI-SIGMA(28) FACTOR ; NEGATIVE REGULATOR ; GENE-EXPRESSION
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000267255200014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375560
专题	武汉病毒研究所
通讯作者	Chen, Shiyun
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ Nottingham, Ctr Biomol Sci, Inst Infect Immun & Inflammat, Nottingham NG7 2UH, England 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ding, Lisha,Wang, Yao,Hu, Yangbo,et al. Functional characterization of flgm in the regulation of flagellar synthesis and motility in yersinia pseudotuberculosis[J]. Microbiology-sgm,2009,155:1890-1900.
APA	Ding, Lisha,Wang, Yao,Hu, Yangbo,Atkinson, Steve,Williams, Paul,&Chen, Shiyun.(2009).Functional characterization of flgm in the regulation of flagellar synthesis and motility in yersinia pseudotuberculosis.Microbiology-sgm,155,1890-1900.
MLA	Ding, Lisha,et al."Functional characterization of flgm in the regulation of flagellar synthesis and motility in yersinia pseudotuberculosis".Microbiology-sgm 155(2009):1890-1900.

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