QQ客服 官方微博 反馈留言

	Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis
	Hu, Yangbo1,3; Wang, Yao1; Ding, Lisha1,3; Lu, Pei1,3; Atkinson, Steve2; Chen, Shiyun1
刊名	Microbiology-sgm
	2009-11-01
卷号	155页码:3622-3631
ISSN号	1350-0872
DOI	10.1099/mic.0.030908-0
通讯作者	Chen, shiyun(sychen@wh.iov.cn)
英文摘要	Ompr has been demonstrated to negatively regulate the expression of the flagellar master operon flhdc in a wide variety of bacterial species. here we report the positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis. a sigma(70)-dependent promoter was identified by primer extension analysis and an active region with two conserved ompr-binding sites around the flhdc promoter was confirmed. to confirm the regulation of flhdc expression by ompr, flhdc as well as the downstream flagellar genes flia, flgd, flga, flgm, flic and flaa were fused to lacz, and decreased expression of all these genes in an ompr mutant (delta ompr) was detected. furthermore, delta ompr was defective in bacterial motility and flagella synthesis. this defect was due to the low level of expression of flhdc in delta ompr since overproduction of flhdc in delta ompr restored bacterial motility. the importance of two conserved ompr-binding sites around the flhdc promoter region in the regulation of flhdc expression by ompr was demonstrated by the fact that mutation of either one or both sites significantly decreased the promoter activity in the wild-type but not in delta ompr. the binding of ompr to these two sites was also demonstrated by dna mobility shift assay. the possible mechanism underlying this positive regulation in y. pseudotuberculosis is discussed. to our knowledge, this is the first report to demonstrate that ompr positively regulates flhdc expression.
WOS关键词	ENTEROHEMORRHAGIC ESCHERICHIA-COLI ; GRAM-NEGATIVE BACTERIA ; H-NS PROTEIN ; MASTER OPERON ; SALMONELLA-TYPHIMURIUM ; FLAGELLAR EXPRESSION ; TRANSCRIPTION FACTOR ; SWIMMING MOTILITY ; BIOFILM FORMATION ; ACETYL PHOSPHATE
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	SOC GENERAL MICROBIOLOGY
WOS记录号	WOS:000272121300017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375558
专题	武汉病毒研究所
通讯作者	Chen, Shiyun
作者单位	1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ Nottingham, Inst Infect Immun & Inflammat, Ctr Biomol Sci, Nottingham NG7 2UH, England 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yangbo,Wang, Yao,Ding, Lisha,et al. Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis[J]. Microbiology-sgm,2009,155:3622-3631.
APA	Hu, Yangbo,Wang, Yao,Ding, Lisha,Lu, Pei,Atkinson, Steve,&Chen, Shiyun.(2009).Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis.Microbiology-sgm,155,3622-3631.
MLA	Hu, Yangbo,et al."Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis".Microbiology-sgm 155(2009):3622-3631.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。