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	Development of monoclonal antibody-coated immunomagnetic beads for separation and detection of norovirus (genogroup ii) in faecal extract samples
	Yao, L.1,2,3; Wu, Q.1; Wang, D.1; Kou, X.1; Zhang, J.1
刊名	Letters in applied microbiology
	2009-08-01
卷号	49期号:2页码:173-178
关键词	Detection Immunomagnetic beads Monoclonal antibody Norovirus Separation
ISSN号	0266-8254
DOI	10.1111/j.1472-765x.2009.02638.x
通讯作者	Wu, q.(wuqp302@yahoo.com.cn)
英文摘要	Aims: the aim of this study is to develop an rt-pcr assay combined with immunomagnetic beads (ims/rt-pcr) coating monoclonal antibody (mab) for separation and detection of norovirus (genogroup ii) in faecal samples. we furthermore compare its detection limits with ims/rt-pcr using polyclonal antibody (pab) and the trizol extraction method followed by rt-pcr (trizol-rt-pcr). methods and results: mab-coated beads and pab-coated beads were added to a series of tenfold dilutions of faecal extract containing norovirus in 1 ml pbs. after incubation and collection, the rna was released by heating from virus separated by beads. the tenfold dilutions of faecal were also extracted with trizol reagent. the rna was used as the template for rt-pcr detection (primers: jv12-jv13). ims/rt-pcr using mab showed an endpoint in the 10(-7) dilution and was 10(2) times more sensitive than ims/rt-pcr using pab and was at least 10(3) times more sensitive than trizol-rt-pcr method. conclusions: ims/rt-pcr using mab proved to be a more sensitive method of noroviruses (nvs) detection than ims/rt-pcr using pab and the trizol-rt-pcr method. significance and impact of the study: this is the first study to detect nvs with ims/rt-pcr using mab, and could serve as a model for future assays when broadly reactive nvs-specific mabs are developed.
WOS关键词	NORWALK-LIKE VIRUSES ; HEPATITIS-A VIRUS ; REVERSE TRANSCRIPTION-PCR ; ROUND-STRUCTURED VIRUSES ; VIRAL GASTROENTERITIS ; WATER SAMPLES ; OUTBREAKS ; IDENTIFICATION ; EPIDEMIOLOGY ; DIAGNOSIS
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
WOS记录号	WOS:000268030100005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375535
专题	武汉病毒研究所
通讯作者	Wu, Q.
作者单位	1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou 510070, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yao, L.,Wu, Q.,Wang, D.,et al. Development of monoclonal antibody-coated immunomagnetic beads for separation and detection of norovirus (genogroup ii) in faecal extract samples[J]. Letters in applied microbiology,2009,49(2):173-178.
APA	Yao, L.,Wu, Q.,Wang, D.,Kou, X.,&Zhang, J..(2009).Development of monoclonal antibody-coated immunomagnetic beads for separation and detection of norovirus (genogroup ii) in faecal extract samples.Letters in applied microbiology,49(2),173-178.
MLA	Yao, L.,et al."Development of monoclonal antibody-coated immunomagnetic beads for separation and detection of norovirus (genogroup ii) in faecal extract samples".Letters in applied microbiology 49.2(2009):173-178.

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