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	Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein
	Chen, Longyan1,2; Wei, Hongping1; Guo, Yongchao1; Cui, Zongqiang1; Zhang, Zhiping1; Zhang, Xian-En1
刊名	Journal of immunological methods
	2009-07-31
卷号	346期号:1-2页码:64-70
关键词	Hantaan virus nucleocapsid protein Gold nanoparticle Immuno-pcr
ISSN号	0022-1759
DOI	10.1016/j.jim.2009.05.007
通讯作者	Wei, hongping(hpwei@wh.iov.cn)
英文摘要	A functionalized gold nanoparticle (gnp) enhanced ultrasensitive immuno-pcr assay (gnp-ipcr on elisa plate), which was modified from the recent developed bio-barcode assay (bca) technique, was developed to detect hantaan virus nucleocapsid protein (hnp). during the assay, the target antigen hnp was captured by a polyclonal antibody coated on elisa microplate wells, followed by adding gnp dually modified with oligonucleotides and a hnp specific monoclonal antibody l13 (mab l13) to form a sandwich immuno-complex. the oligonucleotides on the gnp contained two strands: one as capture dna immobilized on the surface of the gnp through au-s bond and the other as signal amplification dna, which was partially complementary with the capture dna. after the immuno-complex was formed, the signal dna was released by heating, and consequently characterized by pcr/gel electrophoresis and sybr-green real time pcr. the detection limit of this method could reach down to 10 fg/ml for detecting purified hnp in buffers as well as in human serum, which was similar to 7 orders of magnitude more sensitive than that of conventional elisa. the current assay format might be adopted for other proteins that need ultra-high sensitive detection. (c) 2009 elsevier b.v. all rights reserved.
WOS关键词	POLYMERASE-CHAIN-REACTION ; BARCODE AMPLIFICATION ASSAY ; HANTAVIRUS INFECTIONS ; HEMORRHAGIC-FEVER ; RENAL SYNDROME ; SEROLOGICAL ASSAYS ; SURFACE COVERAGE ; RT-PCR ; ANTIGEN ; SENSITIVITY
WOS研究方向	Biochemistry & Molecular Biology ; Immunology
WOS类目	Biochemical Research Methods ; Immunology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000268199900008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375530
专题	武汉病毒研究所
通讯作者	Wei, Hongping
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chen, Longyan,Wei, Hongping,Guo, Yongchao,et al. Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein[J]. Journal of immunological methods,2009,346(1-2):64-70.
APA	Chen, Longyan,Wei, Hongping,Guo, Yongchao,Cui, Zongqiang,Zhang, Zhiping,&Zhang, Xian-En.(2009).Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein.Journal of immunological methods,346(1-2),64-70.
MLA	Chen, Longyan,et al."Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein".Journal of immunological methods 346.1-2(2009):64-70.

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