Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus | |
Song, Jingjiao; Wang, Ranran; Deng, Fei; Wang, Hualin; Hu, Zhihong1 | |
刊名 | Journal of general virology
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2008-09-01 | |
卷号 | 89页码:2331-2338 |
ISSN号 | 0022-1317 |
DOI | 10.1099/vir.0.2008/002352-0 |
通讯作者 | Hu, zhihong(huzh@wh.iov.cn) |
英文摘要 | A combined functional investigation on the four per os infectivity factors (pifs) of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hearnpv) was conducted in this study. hearnpv bacmids with deletions of p74 (ha20), pif1 (ha111), pif2 (ha132) and pif3 (ha98) were constructed individually by homologous recombination in escherichia coli cells. repaired bacmids with respective pifs were also constructed. western blot analyses revealed that all four pifs were structural components of the envelope of hearnpv occlusion-derived virus (odv). electron microscopy showed that deletion of the pifs did not have any obvious effects on the morphology of the occlusion bodies (obs). bioassay analyses indicated that deletion of any of the above pifs resulted in loss of oral infectivity of obs. the mixtures of the four pif-deletion mutants also resulted in deficiency of oral infectivity, implying that the four pifs must be structural components of the same odv to accomplish their function. repairing of the respective genes into the pif-deletion bacmids could rescue the oral infectivity of the pit-deletion viruses. calcofluor, which can damage the peritrophic membrane (pm), could not rescue the defects of the oral infectivity of the pif-deletion viruses, indicating that the pm is not likely to be the functional target of the pifs. |
WOS关键词 | OCCLUSION-DERIVED VIRUS ; HELIOTHIS-VIRESCENS LARVAE ; NUCLEAR POLYHEDROSIS-VIRUS ; ENVELOPE FUSION PROTEIN ; BACULOVIRUS ; P74 ; MIDGUT ; ENTRY ; IDENTIFICATION ; INSECTS |
WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
WOS类目 | Biotechnology & Applied Microbiology ; Virology |
语种 | 英语 |
出版者 | SOC GENERAL MICROBIOLOGY |
WOS记录号 | WOS:000259285600030 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2375515 |
专题 | 武汉病毒研究所 |
通讯作者 | Hu, Zhihong |
作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Jingjiao,Wang, Ranran,Deng, Fei,et al. Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus[J]. Journal of general virology,2008,89:2331-2338. |
APA | Song, Jingjiao,Wang, Ranran,Deng, Fei,Wang, Hualin,&Hu, Zhihong.(2008).Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.Journal of general virology,89,2331-2338. |
MLA | Song, Jingjiao,et al."Functional studies of per os infectivity factors of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus".Journal of general virology 89(2008):2331-2338. |
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