QQ客服 官方微博 反馈留言

	Enhancement of heavy metal accumulation by tissue specific co-expression of iaam and acc deaminase genes in plants
	Zhang, Yong1,2; Zhao, Lihong1; Wang, Yao1; Yang, Baoyu1; Chen, Shiyun1
刊名	Chemosphere
	2008-06-01
卷号	72期号:4页码:564-571
关键词	Acc deaminase Iaam Heavy metals Phytoremediation Tissue specific co-expression Xylem-specific promoter
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2008.03,043
通讯作者	Chen, shiyun(sychen@wh.iov.cn)
英文摘要	1-aminocyclopropane deaminase (acc) and tryptophan monooxygenase are two enzymes involved in plant senescence-inhibiting and growth-promoting regulation, respectively. in this study, two binary vectors were constructed in which the agrobacterium iaam gene was under the transcriptional control of a xylem-specific glycine-rich protein promoter alone, or co-expressed with the bacterial acc deaminase gene, which was driven by the constitutive camv 35s promoter. transgenic petunia shoots co-expressing both genes were able to root on medium supplemented with 7.5 mg 1(-1) cocl2. when t, transgenic tobacco plants were grown in sand supplemented with cu2+ and co2+, tissue specific co-expression of both iaam and acc deaminase genes showed faster growth with larger biomass with a more extensive root system, and accumulated a greater amount of heavy metals than the empty vector control plants. when t, transgenic tobacco plants were grown in soil watered with different concentrations of cuso4, xylem specific expression of the iaam gene caused the accumulation of more cu2+ than the empty vector control at lower cuso4 concentrations, but showed severe toxic symptoms at concentration of 100 mg 1(-1) cuso4. t, transgenic plants co-expressing both genes accumulated more heavy metals into the plant shoots and can tolerate cuso4 at 150 mg 1(-1). in addition, plants co-expressing these two genes can grow well in a complex contaminated soil containing both inorganic and organic pollutants, while the growth of the control plants was greatly inhibited. (c) 2008 elsevier ltd. all rights reserved.
WOS关键词	GROWTH-PROMOTING BACTERIA ; CANOLA BRASSICA-NAPUS ; YA-ER LAKE ; TRANSGENIC PLANTS ; 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE ; PHYTOCHELATIN SYNTHASE ; ENGINEERING TOLERANCE ; THLASPI-CAERULESCENS ; CONTAMINATED SOIL ; ETHYLENE LEVELS
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000257198900007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375505
专题	武汉病毒研究所
通讯作者	Chen, Shiyun
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yong,Zhao, Lihong,Wang, Yao,et al. Enhancement of heavy metal accumulation by tissue specific co-expression of iaam and acc deaminase genes in plants[J]. Chemosphere,2008,72(4):564-571.
APA	Zhang, Yong,Zhao, Lihong,Wang, Yao,Yang, Baoyu,&Chen, Shiyun.(2008).Enhancement of heavy metal accumulation by tissue specific co-expression of iaam and acc deaminase genes in plants.Chemosphere,72(4),564-571.
MLA	Zhang, Yong,et al."Enhancement of heavy metal accumulation by tissue specific co-expression of iaam and acc deaminase genes in plants".Chemosphere 72.4(2008):564-571.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。