| Electroporation at low voltages enables dna vaccine to provide protection against a lethal h5n1 avian influenza virus challenge in mice | |
| Zhou, Yu1; Fang, Fang1; Chen, Jianjun2; Wang, Huadong1; Chang, Haiyan1; Yang, Zhongdong3; Chen, Ze1,2,3 | |
| 刊名 | Intervirology
 |
| 2008 | |
| 卷号 | 51期号:4页码:241-246 |
| 关键词 | Dna vaccine Electroporation H5n1 Hemagglutinin |
| ISSN号 | 0300-5526 |
| DOI | 10.1159/000156483 |
| 通讯作者 | Chen, ze(chenze2005@hotmail.com) |
| 英文摘要 | Objective: electroporation has been proved to enhance the efficacy of intramuscular delivery of dna. however, the process of electroporation causes pain and discomfort to the patient receiving the treatment. higher the electroporation voltages inflict greater pain, and this limits the circumstances in which the technique can be used clinically. the voltages generally used for electroporation in animals range from 100 to 1,200 v/cm. we studied the effect of dna vaccination when electroporation was performed at lower voltages. methods: balb/c mice were immunized twice by electroporation, at a 3-week interval, with h5n1 virus hemagglutinin (ha) dna. one week after the booster had been given, the mice were challenged with a lethal dose of mouse-adapted h5n1 virus. the immune effects of ha dna were evaluated by the survival rate, lung virus titer, bodyweight change and antibody titer of the mice. results: the higher the voltage used, the more able were the mice to survive the challenge. however, a significant degree of protection could also be achieved with a voltage as low as 5 v. when electroporation was performed at a voltage of 10 v, an immunization amount of 5 mu g is enough for ha dna to provide effective protection. conclusion: low-voltage electroporation can induce immunity and protect mice effectively. there is, therefore, the potential to reduce the voltages currently used for dna electroporation. copyright (c) 2008 s. karger ag, basel |
| WOS关键词 | IN-VIVO ELECTROPORATION ; GENE-TRANSFER ; PLASMID DNA ; SKELETAL-MUSCLE ; IMMUNE-RESPONSES ; A VIRUS ; TISSUE DISTRIBUTION ; ELECTRIC PULSES ; VIRAL PROTEIN ; T-CELLS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | KARGER |
| WOS记录号 | WOS:000260892200005 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375500 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Ze |
| 作者单位 | 1.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China 3.Shanghai Inst Biol Prod, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yu,Fang, Fang,Chen, Jianjun,et al. Electroporation at low voltages enables dna vaccine to provide protection against a lethal h5n1 avian influenza virus challenge in mice[J]. Intervirology,2008,51(4):241-246. |
| APA | Zhou, Yu.,Fang, Fang.,Chen, Jianjun.,Wang, Huadong.,Chang, Haiyan.,...&Chen, Ze.(2008).Electroporation at low voltages enables dna vaccine to provide protection against a lethal h5n1 avian influenza virus challenge in mice.Intervirology,51(4),241-246. |
| MLA | Zhou, Yu,et al."Electroporation at low voltages enables dna vaccine to provide protection against a lethal h5n1 avian influenza virus challenge in mice".Intervirology 51.4(2008):241-246. |
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