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	One-step label-free optical genosensing system for sequence-specific dna related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods
	He, Wei4; Huang, Cheng Zhi1; Li, Yuan Fang1; Xie, Jian Ping2; Yang, Rong Ge3; Zhou, Pei Fu2; Wang, Jian4
刊名	Analytical chemistry
	2008-11-15
卷号	80期号:22页码:8424-8430
ISSN号	0003-2700
DOI	10.1021/ac801005d
通讯作者	Huang, cheng zhi(chengzhi@swu.edu.cn)
英文摘要	A one-step label-free optical genosensing method has been developed in this contribution by taking short dna target with its sequence related to the human immunodeficiency virus type 1 (hiv-1) as an example. by employing anisotropic nonspherical and positively charged gold nanorods (au-nrs) as the recognition platform, which show high stability against aggregation under high ionic strength conditions without any additional stable reagent, we found that the addition of target dna to the mixture of nonmodified au-nrs suspension and label-free probe dna in high ionic strength buffer leads to a color change from red to fight purple in less than 5 min, displaying strong plasmon resonance light scattering (prls) signals. mechanism investigations showed that the strong prls signals should be ascribed to the aggregation ofau-nrs induced by the formed double-stranded oligonucleotides (dsdna) from the hybridization of target dna with probe dna. with the prls signals, we monitored the hybridization process of a 2 1-mer single-stranded oligonucleotide (ssdna) from the fhv-1 u5 long terminal repeat (ltr) sequence with its complementary oligonucleotide and detected the effect of single-base-pair mismatches. two polymerase chain reaction (pcr) amplicon artificial samples derived from mycobacterium tuberculosis glms and genes encoding for bacillus glucanase and an hiv-1 ltr sample isolated from hiv-1-positive blood were detected with satisfactory results, showing that the present method has simplicity, sensitivity, specificity, and reliability for sequence-specific dna detection related to the hiv gene.
WOS关键词	SINGLE-STRANDED-DNA ; ENHANCED RAMAN-SCATTERING ; COLORIMETRIC DETECTION ; SILVER NANOPARTICLES ; CATIONIC SURFACTANTS ; METAL NANOPARTICLES ; LATEX-PARTICLES ; ABSORPTION ; ADSORPTION ; SHAPE
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000260910900009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375437
专题	武汉病毒研究所
通讯作者	Huang, Cheng Zhi
作者单位	1.Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China 2.Southwest Univ, Educ Minist, Key Lab Luminescence & Real Time Anal, Coll Life Sci,Inst Modern Biopharmaceut, Chongqing 400715, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, AIDS & HIV Res Grp, Wuhan 430071, Peoples R China 4.Southwest Univ, Coll Chem & Chem Engn, Chongqing 400715, Peoples R China
推荐引用方式 GB/T 7714	He, Wei,Huang, Cheng Zhi,Li, Yuan Fang,et al. One-step label-free optical genosensing system for sequence-specific dna related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods[J]. Analytical chemistry,2008,80(22):8424-8430.
APA	He, Wei.,Huang, Cheng Zhi.,Li, Yuan Fang.,Xie, Jian Ping.,Yang, Rong Ge.,...&Wang, Jian.(2008).One-step label-free optical genosensing system for sequence-specific dna related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods.Analytical chemistry,80(22),8424-8430.
MLA	He, Wei,et al."One-step label-free optical genosensing system for sequence-specific dna related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods".Analytical chemistry 80.22(2008):8424-8430.

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