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	Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus
	Zheng, Dasheng; Valdez-Cruz, Norma Adriana; Armengol, Gemma; Sevrez, Chloe; Munoz-Olaya, Jose Maurilio; Yuan, Zhiming; Orduz, Sergio; Crickmore, Neil
刊名	Current microbiology
	2007
卷号	54期号:1页码:58-62
ISSN号	0343-8651
DOI	10.1007/s00284-006-0352-7
通讯作者	Crickmore, neil(n.crickmore@sussex.ac.uk)
英文摘要	The cyt1aa gene from bacillus thuringiensis subsp. israelensis (bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against bacilli insecticides, was introduced into the aquatic gram-negative bacterium asticcacaulis excentricus alongside the cry11aa gene. the genes were introduced as an operon, but although mrna was detected for both genes, no cyt1aa toxin was detected. both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. recombinant a. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of culex quinquefasciatus as transformants expressing just cry11aa.
WOS关键词	CULEX-QUINQUEFASCIATUS DIPTERA ; AGMENELLUM-QUADRUPLICATUM PR-6 ; LARVICIDAL ACTIVITY ; EXPRESSION ; GENES ; RESISTANCE ; SPHAERICUS ; CULICIDAE ; STRAIN ; TOXICITY
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000242969100012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375385
专题	武汉病毒研究所
通讯作者	Crickmore, Neil
作者单位	1.Univ Sussex, Sch Life Sci, Brighton, E Sussex, England 2.Corp Invest Biol, Unidad Biotecnol & Control Biol, Medellin, Colombia 3.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Dasheng,Valdez-Cruz, Norma Adriana,Armengol, Gemma,et al. Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus[J]. Current microbiology,2007,54(1):58-62.
APA	Zheng, Dasheng.,Valdez-Cruz, Norma Adriana.,Armengol, Gemma.,Sevrez, Chloe.,Munoz-Olaya, Jose Maurilio.,...&Crickmore, Neil.(2007).Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus.Current microbiology,54(1),58-62.
MLA	Zheng, Dasheng,et al."Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus".Current microbiology 54.1(2007):58-62.

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