Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus | |
Zheng, Dasheng; Valdez-Cruz, Norma Adriana; Armengol, Gemma; Sevrez, Chloe; Munoz-Olaya, Jose Maurilio; Yuan, Zhiming; Orduz, Sergio; Crickmore, Neil | |
刊名 | Current microbiology
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2007 | |
卷号 | 54期号:1页码:58-62 |
ISSN号 | 0343-8651 |
DOI | 10.1007/s00284-006-0352-7 |
通讯作者 | Crickmore, neil(n.crickmore@sussex.ac.uk) |
英文摘要 | The cyt1aa gene from bacillus thuringiensis subsp. israelensis (bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against bacilli insecticides, was introduced into the aquatic gram-negative bacterium asticcacaulis excentricus alongside the cry11aa gene. the genes were introduced as an operon, but although mrna was detected for both genes, no cyt1aa toxin was detected. both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. recombinant a. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of culex quinquefasciatus as transformants expressing just cry11aa. |
WOS关键词 | CULEX-QUINQUEFASCIATUS DIPTERA ; AGMENELLUM-QUADRUPLICATUM PR-6 ; LARVICIDAL ACTIVITY ; EXPRESSION ; GENES ; RESISTANCE ; SPHAERICUS ; CULICIDAE ; STRAIN ; TOXICITY |
WOS研究方向 | Microbiology |
WOS类目 | Microbiology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000242969100012 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2375385 |
专题 | 武汉病毒研究所 |
通讯作者 | Crickmore, Neil |
作者单位 | 1.Univ Sussex, Sch Life Sci, Brighton, E Sussex, England 2.Corp Invest Biol, Unidad Biotecnol & Control Biol, Medellin, Colombia 3.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Dasheng,Valdez-Cruz, Norma Adriana,Armengol, Gemma,et al. Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus[J]. Current microbiology,2007,54(1):58-62. |
APA | Zheng, Dasheng.,Valdez-Cruz, Norma Adriana.,Armengol, Gemma.,Sevrez, Chloe.,Munoz-Olaya, Jose Maurilio.,...&Crickmore, Neil.(2007).Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus.Current microbiology,54(1),58-62. |
MLA | Zheng, Dasheng,et al."Co-expression of the mosquitocidal toxins cyt1aa and cry11aa from bacillus thuringiensis subsp israelensis in asticcacaulis excentricus".Current microbiology 54.1(2007):58-62. |
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