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	Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus
	Liu, Li; Fang, Qing; Deng, Fei; Wang, Hanzhong; Yi, Christopher E.; Ba, Lei; Yu, Wenjie; Lin, Richard D.; Li, Taisheng; Hu, Zhihong
刊名	Journal of virology
	2007-05-01
卷号	81期号:9页码:4694-4700
ISSN号	0022-538X
DOI	10.1128/jvi.02389-06
通讯作者	Chen, zhiwei(zchen@adarc.org)
英文摘要	The severe acute respiratory syndrome (sars) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. coronavirus (cov) found in naturally infected palm civet (civet-cov) represents the closest genetic relative to sars-cov, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. studies indicate that the receptor binding domain (rbd) of the sars-cov spike (s) glycoprotein contains major determinants for viral entry and neutralization. we aim to characterize the impact of natural mutations within the rbds of civet-covs on viral entry and cross-neutralization. in this study, the s glycoprotein genes were recovered from naturally infected civets in central china (hubei province), extending the geographic distribution of civet-cov beyond the southeastern province of guangdong. moreover, pseudoviruses generated in our laboratory with four civet s genes, each with a distinct rbd, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of sars-cov. these four civet s genes were also constructed as dna vaccines to immunize mice. immunized sera elicited against most civet s glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing sars-cov and vice versa. convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. our findings suggest that the design of sars vaccine should consider not only preventing the reemergence of sars-cov but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet covs of broad geographic origin.
WOS关键词	HUMAN MONOCLONAL-ANTIBODY ; SARS-CORONAVIRUS ; PROTECTIVE IMMUNITY ; MOLECULAR EVOLUTION ; PROTEIN ; VIRUS ; INFECTION ; VACCINE ; MICE ; IMMUNOGENICITY
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000246501900032
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375355
专题	武汉病毒研究所
通讯作者	Chen, Zhiwei
作者单位	1.Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Med Sci, AIDS Res Ctr, Beijing 100730, Peoples R China 4.Peking Union Med Coll, Beijing 100730, Peoples R China
推荐引用方式 GB/T 7714	Liu, Li,Fang, Qing,Deng, Fei,et al. Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus[J]. Journal of virology,2007,81(9):4694-4700.
APA	Liu, Li.,Fang, Qing.,Deng, Fei.,Wang, Hanzhong.,Yi, Christopher E..,...&Chen, Zhiwei.(2007).Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus.Journal of virology,81(9),4694-4700.
MLA	Liu, Li,et al."Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus".Journal of virology 81.9(2007):4694-4700.

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