| Transduction of avian cells with recombinant baculovirus | |
| Song, Jianhua; Liang, Changyong; Chen, Xinwen | |
| 刊名 | Journal of virological methods
 |
| 2006-08-01 | |
| 卷号 | 135期号:2页码:157-162 |
| 关键词 | Baculovirus Avian Transduction |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2006.02.013 |
| 通讯作者 | Chen, xinwen(chenxw@pentium.whiov.ac.cn) |
| 英文摘要 | Transduction of chicken and duck cells was examined by ac-cmv-egfp, a recombinant baculovirus capable of expressing an egfp reporter gene under the control of the cmv promoter. the results showed that chicken and duck cells were transducible, as demonstrated by a flow cytometry assay. the transduction efficiency of duck cells was higher than that of chicken cells. the addition of histone deacetylase inhibitor sodium butyrate enhanced the expression levels of egfp both in chicken and duck cells. ac-cmv-egfp is capable of transducing genes into a variety of chicken cells in organs such as liver, lung and kidney. of three cells from different organs tested, the highest transduction was observed in lung cells (49.8%), followed by kidney cells (44%) and liver cells (43%). only in chicken liver cells acmnpv transduction was in a dose-dependent manner. it also showed that baculovirus enters the avian cells by endocytosis and is released into the cytoplasm by acid-induced fusion. (c) 2006 published by elsevier b.v. |
| WOS关键词 | EFFICIENT GENE-TRANSFER ; MAMMALIAN-CELLS ; SODIUM-BUTYRATE ; TRANSGENIC MICE ; EXPRESSION ; VECTORS ; INFECTION ; INFLUENZA ; ENTRY ; PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000238965800004 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375333 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xinwen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Jianhua,Liang, Changyong,Chen, Xinwen. Transduction of avian cells with recombinant baculovirus[J]. Journal of virological methods,2006,135(2):157-162. |
| APA | Song, Jianhua,Liang, Changyong,&Chen, Xinwen.(2006).Transduction of avian cells with recombinant baculovirus.Journal of virological methods,135(2),157-162. |
| MLA | Song, Jianhua,et al."Transduction of avian cells with recombinant baculovirus".Journal of virological methods 135.2(2006):157-162. |
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