| Quantitative relationship of two viruses (mrnv and xsv) in white-tail disease of macrobrachium rosenbergii | |
| Zhang, Huajun; Wang, Hanmin; Yuan, Junfa; Li, Lijuan; Zhang, Jianhong; Bonami, Jean-Robert; Shi, Zhengli | |
| 刊名 | Diseases of aquatic organisms
 |
| 2006-07-11 | |
| 卷号 | 71期号:1页码:11-17 |
| 关键词 | Macrobrachium rosenbergii Nodavirus Extra small virus Real-time rt-pcr White-tail disease |
| ISSN号 | 0177-5103 |
| 通讯作者 | Shi, zhengli(zlshi@wh.iov.cn) |
| 英文摘要 | Macrobrachium rosenbergii nodavirus (mrnv) and extra small virus (xsv) were purified from diseased freshwater prawns m rosenbergii and used to infect healthy post-larvae (pl) by an immersion method. three groups of prawns were challenged with various combined doses of mrnv and xsv. signs of white-tail disease (wtd) were observed in groups 1 and 2, which had been challenged with combinations containing relatively high proportions of mrnv and low proportions of xsv. by contrast there was little sign of wtd in group 3, which had been challenged with a higher proportion of xsv than mrnv. a 2-step taqman real-time rt-pcr was developed and applied to quantify viral copy numbers in each challenged pl. results showed that genomic copies of both viruses were much higher in groups 1 and 2 than they were in group 3, indicating that mrnv plays a key role in wtd of m rosenbergii. the linear correlation between mrnv and xsv genome copies in infected prawns demonstrated that xsv is a satellite virus, dependent on mrnv, but its role in pathogenicity of wtd remains unclear. |
| WOS关键词 | FRESH-WATER PRAWN ; EXTRA SMALL VIRUS ; TOBACCO MOSAIC-VIRUS ; HELPER TOBAMOVIRUSES ; SATELLITE VIRUS ; NECROSIS VIRUS ; DE-MAN ; NODAVIRUS ; ASSAY |
| WOS研究方向 | Fisheries ; Veterinary Sciences |
| WOS类目 | Fisheries ; Veterinary Sciences |
| 语种 | 英语 |
| 出版者 | INTER-RESEARCH |
| WOS记录号 | WOS:000240510300002 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375330 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Zhengli |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Montpellier 2, CNRS, ECOLAG, UMR5119, Montpellier, France |
| 推荐引用方式 GB/T 7714 | Zhang, Huajun,Wang, Hanmin,Yuan, Junfa,et al. Quantitative relationship of two viruses (mrnv and xsv) in white-tail disease of macrobrachium rosenbergii[J]. Diseases of aquatic organisms,2006,71(1):11-17. |
| APA | Zhang, Huajun.,Wang, Hanmin.,Yuan, Junfa.,Li, Lijuan.,Zhang, Jianhong.,...&Shi, Zhengli.(2006).Quantitative relationship of two viruses (mrnv and xsv) in white-tail disease of macrobrachium rosenbergii.Diseases of aquatic organisms,71(1),11-17. |
| MLA | Zhang, Huajun,et al."Quantitative relationship of two viruses (mrnv and xsv) in white-tail disease of macrobrachium rosenbergii".Diseases of aquatic organisms 71.1(2006):11-17. |
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