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	Effects of gastrin 17 on beta-catenin/tcf-4 pathway in colo320wt colon cancer cells
	Cao, Jun; Yu, Lie-Ping; Liu, Chao-Hong; Zhou, Lan; Yu, Hong-Gang
刊名	World journal of gastroenterology
	2006-12-14
卷号	12期号:46页码:7482-7487
关键词	Gastrin17 Cholecystokinin-2 receptor Colorectal carcinoma Beta-catenin/tcf-4 pathway
ISSN号	1007-9327
通讯作者	Yu, hong-gang(streamupdown@yahoo.com)
英文摘要	Aim: to explore the effect of gastrin 17 (g17) on beta-catenin/t cell factor-4 (tcf-4) signaling in colonic cancer cell line colo320wt. methods: the pcr3.1/gr plasmid, which expresses gastrin receptor, cholecystokinin-2 receptor (cck-2r), was transfected into a colonic cancer cell line colo320 by lipofectamine (tm) 2000 and the stably expressing cck-2r clones were screened by g418. the expression levels of gastrin receptor in the colo320 and the transfected colo320wt cell line were assayed by rt-pcr. colo320wt cells were treated with g17 in a time-dependent manner (0, 1, 6, 12, 24 and 48 h), then with l365,260 (gastrin(17) receptor blocker) for 30 min, and with g17 again for 12 h or l365,260 for 12 h. expression levels of beta-catenin in a tx-100 soluble fraction and tx-100 insoluble fraction of colo320wt cells treated with g17 were detected by co-immuniprecipation and western blot. immunocytochemistry was used to examine the distribution of beta-catenin in colowt320 cells. expression levels of c-myc and cyclin d1 in colo320wt cells treated with g17 were assayed by western blot. results: expression levels of beta-catenin in the tx-100 solution fraction decreased apparently in a time-dependent fashion and reached the highest level after g17 treatment for 12 h, while expression levels of beta-catenin in the tx-100 insoluble fraction were just on the contrary. immunocytochemistry showed that beta-catenin was translocated from the cell membranes into the cytoplasm and nucleus under g17 treatment. expression levels of c-myc and cyclin d1 in the g17-treated colo320wt cells were markedly higher compared to the untreated colo320wt cells. in addition, the aforementioned g17-stimulated responses were blocked by l365,260. conclusion: gastrin17 activates beta-catenin/tcf-4 signaling in colo320wt cells, thereby leading to overexpression of c-myc and cyclin d1. (c) 2006 the wjg press. all rights reserved.
WOS关键词	INTEGRIN-LINKED KINASE ; CYCLIN D1 GENE ; E-CADHERIN ; BETA-CATENIN ; COLORECTAL-CANCER ; ACTIVATION ; EXPRESSION ; ADHESION ; RECEPTOR ; COMPLEX
WOS研究方向	Gastroenterology & Hepatology
WOS类目	Gastroenterology & Hepatology
语种	英语
出版者	W J G PRESS
WOS记录号	WOS:000242962000014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375305
专题	武汉病毒研究所
通讯作者	Yu, Hong-Gang
作者单位	1.Wuhan Univ, Renmin Hosp, Dept Gastroenterol, Wuhan 430060, Hubei Province, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430060, Hubei Province, Peoples R China 3.Hubei Coll Tradit Chinese Med, Tradit Chinese Med Expt Ctr, Wuhan 4300061, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Cao, Jun,Yu, Lie-Ping,Liu, Chao-Hong,et al. Effects of gastrin 17 on beta-catenin/tcf-4 pathway in colo320wt colon cancer cells[J]. World journal of gastroenterology,2006,12(46):7482-7487.
APA	Cao, Jun,Yu, Lie-Ping,Liu, Chao-Hong,Zhou, Lan,&Yu, Hong-Gang.(2006).Effects of gastrin 17 on beta-catenin/tcf-4 pathway in colo320wt colon cancer cells.World journal of gastroenterology,12(46),7482-7487.
MLA	Cao, Jun,et al."Effects of gastrin 17 on beta-catenin/tcf-4 pathway in colo320wt colon cancer cells".World journal of gastroenterology 12.46(2006):7482-7487.

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