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White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus
Bonami, JR; Shi, Z; Qian, D; Sri Widada, J
刊名Journal of fish diseases
2005
卷号28期号:1页码:23-31
关键词Macrobrachium rosenbergii Mrnv Nodavirus Rna viruses Satellite virus-like particle White tail disease Xsv
ISSN号0140-7775
通讯作者Bonami, jr(bonami@univ-montp2.fr)
英文摘要White tail disease of the farmed freshwater prawn, macrobrachium rosenbergii, is the cause of mortalities in the french west indies, china and india. two different sized particles, both developing in the cytoplasm of target cells, are found associated with diseased animals. these two viruses were separated, purified and subsequently characterized. the larger one, called mrnv, is icosahedral in shape and 27 nm in diameter. its genome is composed of two fragments of linear single-stranded rna (ss-rna), of 2.9 and 1.3 kb, respectively and its capsids exhibited a single polypeptide of 43 kda. these characteristics and the partial sequence of a cloned fragment of rna-1 suggest this agent is a member of the family nodaviridae, but with differences from both the genera alphanodavirus and betanodavirus. the smaller virus, named xsv, is icosahedral in shape, 15 nm in diameter, possesses a linear ss-rna genome of about 0.9 kb, and its capsid exhibits two polypeptides of 16 and 17 kda, respectively. the relationships between these two viruses remain unknown.
WOS关键词EXTRA SMALL VIRUS ; DICENTRARCHUS-LABRAX ; DOT-BLOT ; RT-PCR ; HYBRIDIZATION ; MORTALITIES ; PARTICLES ; GENOME ; XSV
WOS研究方向Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
出版者BLACKWELL PUBLISHING LTD
WOS记录号WOS:000226400900004
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2375234
专题武汉病毒研究所
通讯作者Bonami, JR
作者单位1.Univ Montpellier 2, ECOLAG, CNRS, UMR5119, F-34095 Montpellier 05, France
2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Peoples R China
3.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China
推荐引用方式
GB/T 7714
Bonami, JR,Shi, Z,Qian, D,et al. White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus[J]. Journal of fish diseases,2005,28(1):23-31.
APA Bonami, JR,Shi, Z,Qian, D,&Sri Widada, J.(2005).White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus.Journal of fish diseases,28(1),23-31.
MLA Bonami, JR,et al."White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus".Journal of fish diseases 28.1(2005):23-31.
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