White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus | |
Bonami, JR; Shi, Z; Qian, D; Sri Widada, J | |
刊名 | Journal of fish diseases
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2005 | |
卷号 | 28期号:1页码:23-31 |
关键词 | Macrobrachium rosenbergii Mrnv Nodavirus Rna viruses Satellite virus-like particle White tail disease Xsv |
ISSN号 | 0140-7775 |
通讯作者 | Bonami, jr(bonami@univ-montp2.fr) |
英文摘要 | White tail disease of the farmed freshwater prawn, macrobrachium rosenbergii, is the cause of mortalities in the french west indies, china and india. two different sized particles, both developing in the cytoplasm of target cells, are found associated with diseased animals. these two viruses were separated, purified and subsequently characterized. the larger one, called mrnv, is icosahedral in shape and 27 nm in diameter. its genome is composed of two fragments of linear single-stranded rna (ss-rna), of 2.9 and 1.3 kb, respectively and its capsids exhibited a single polypeptide of 43 kda. these characteristics and the partial sequence of a cloned fragment of rna-1 suggest this agent is a member of the family nodaviridae, but with differences from both the genera alphanodavirus and betanodavirus. the smaller virus, named xsv, is icosahedral in shape, 15 nm in diameter, possesses a linear ss-rna genome of about 0.9 kb, and its capsid exhibits two polypeptides of 16 and 17 kda, respectively. the relationships between these two viruses remain unknown. |
WOS关键词 | EXTRA SMALL VIRUS ; DICENTRARCHUS-LABRAX ; DOT-BLOT ; RT-PCR ; HYBRIDIZATION ; MORTALITIES ; PARTICLES ; GENOME ; XSV |
WOS研究方向 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS类目 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
语种 | 英语 |
出版者 | BLACKWELL PUBLISHING LTD |
WOS记录号 | WOS:000226400900004 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2375234 |
专题 | 武汉病毒研究所 |
通讯作者 | Bonami, JR |
作者单位 | 1.Univ Montpellier 2, ECOLAG, CNRS, UMR5119, F-34095 Montpellier 05, France 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Peoples R China 3.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Bonami, JR,Shi, Z,Qian, D,et al. White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus[J]. Journal of fish diseases,2005,28(1):23-31. |
APA | Bonami, JR,Shi, Z,Qian, D,&Sri Widada, J.(2005).White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus.Journal of fish diseases,28(1),23-31. |
MLA | Bonami, JR,et al."White tail disease of the giant freshwater prawn, macrobrachium rosenbergii: separation of the associated virions and characterization of mrnv as a new type of nodavirus".Journal of fish diseases 28.1(2005):23-31. |
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