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	Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10)
	Seng, EK; Fang, Q; Sin, YM; Lam, TJ
刊名	Archives of virology
	2005-10-01
卷号	150期号:10页码:2021-2036
ISSN号	0304-8608
DOI	10.1007/s00705-005-0550-9
通讯作者	Seng, ek(dbssek@nus.edu.sg)
英文摘要	Genome segment 10 (s10) of threadfin aquareovirus (tfv) was cloned, sequenced, analyzed and found to be 987 bp long encoding a protein of 298 aa with a predicted molecular mass of 32.0 kda. the tfv s10 gene possesses terminal motifs, (5' gtttta and attcatc 3') which are also conserved in the s6 and s11 tfv gene segments. sequence comparison revealed that the tfv s10 gene was similar to the striped bass reovirus (sbr) vp7 outer capsid protein (ocp). a conserved putative zinc-finger motif, cchc, present in the mammalian reovirus (mrv) delta 3 protein, was identified in tfv and other aquareovirus vp7 protein. phylogenetic analysis of the tfv vp7 protein indicated that tfv is closely related to sbr and chum salmon reovirus (csv) and possibly belong to the same species aquareovirus a as sbr and csv. the tfv vp7 protein was expressed in e. coli, purified and injected into mice. serum specific antibodies were generated, however, the serum showed weak neutralizing activity. in contrast, co-incubation of this serum with another serum obtained from mice immunized with another ocp encoded by the tfv s6 gene segment resulted in a highly elevated antibody neutralization titer.
WOS关键词	RNA BLOT HYBRIDIZATION ; SEQUENCE-ANALYSIS ; ROTAVIRUS ANTIGENICITY ; REOVIRUS ; FISH ; GENOGROUP ; VIRUSES ; VP7 ; IDENTIFICATION ; GLYCOSYLATION
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000232030100007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375216
专题	武汉病毒研究所
通讯作者	Seng, EK
作者单位	1.Natl Univ Singapore, Dept Biol Sci, Virol Lab 05 02, Singapore 117543, Singapore 2.CAS, Wuhan Inst Virol, Wuhan, Peoples R China 3.Teo Way Yong & Sons Pte Ltd, Singapore Fish Breeding & Immunizat Ctr, Singapore, Singapore
推荐引用方式 GB/T 7714	Seng, EK,Fang, Q,Sin, YM,et al. Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10)[J]. Archives of virology,2005,150(10):2021-2036.
APA	Seng, EK,Fang, Q,Sin, YM,&Lam, TJ.(2005).Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10).Archives of virology,150(10),2021-2036.
MLA	Seng, EK,et al."Molecular characterization of a major outer capsid protein encoded by the threadfin aquareovirus (tfv) gene segment 10 (s10)".Archives of virology 150.10(2005):2021-2036.

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