QQ客服 官方微博 反馈留言

	Characterization of the rna-binding domain in the dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44
	Zhao, SL; Liang, CY; Zhang, WJ; Tang, XC; Peng, HY
刊名	Virus research
	2005-12-01
卷号	114期号:1-2页码:80-88
ISSN号	0168-1702
DOI	10.1016/j.virusres.2005.06.002
通讯作者	Zhao, sl(sdzll79@sohu.com)
英文摘要	Dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv-1) belongs to the cypovirus genus in the reoviridae family. the orf of genome segment 8 (s8) of dpcpv-1 was cloned into vector pmal-c2x and used to express a 44 kda protein (p44) in e. coli, which was detected by western blotting. the gel mobility shift assays showed that p44 had ssrna-binding activity. competitive assay indicated that this protein only bind to ssrna and could not interact with dna and dsrna. the binding of p44 to ssrna is sequence non-specific. to identify the domain(s) important for rna binding of the protein, a number of deletions were made. these truncated proteins were expressed in e. coli and purified. the affinity of each truncated protein towards ssrna was then assayed by electrophoretic mobility shift assays and northwestern blot. the results indicated that glutamic acid-rich domain in the central region of p44 from residues 104 to 201 was the ssrna-binding domain. (c) 2005 elsevier b.v. all rights reserved.
WOS关键词	BOMBYX-MORI CYPOVIRUS-1 ; SINGLE-STRANDED RNA ; NUCLEOTIDE-SEQUENCE ; MOLECULAR CHARACTERIZATION ; BLUETONGUE VIRUS ; GENOME SEGMENTS ; RECOGNITION ; GENE ; CLASSIFICATION ; EXPRESSION
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000233645100010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375215
专题	武汉病毒研究所
通讯作者	Zhao, SL
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Lab Biol Control, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, SL,Liang, CY,Zhang, WJ,et al. Characterization of the rna-binding domain in the dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44[J]. Virus research,2005,114(1-2):80-88.
APA	Zhao, SL,Liang, CY,Zhang, WJ,Tang, XC,&Peng, HY.(2005).Characterization of the rna-binding domain in the dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44.Virus research,114(1-2),80-88.
MLA	Zhao, SL,et al."Characterization of the rna-binding domain in the dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44".Virus research 114.1-2(2005):80-88.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。