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	Development and evaluation of a multitarget real-time taqman reverse transcription-pcr assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets
	Hu, WQ; Bai, BK; Hu, ZH; Chen, Z; An, XF; Tang, LJ; Yang, JH; Wang, HL; Wang, HZ
刊名	Journal of clinical microbiology
	2005-05-01
卷号	43期号:5页码:2041-2046
ISSN号	0095-1137
DOI	10.1128/jcm.43.5.2041-2046.2005
通讯作者	Wang, hz()
英文摘要	Severe acute respiratory syndrome (sars)-associated coronavirus (sars-cov) is the etiological agent of sars. it is believed that sars-cov originates from wild animals. we have developed a multitarget real-time taqman reverse transcription-pcr (rt-pcr) assay for the quantitative detection of sars-cov. the sequences of the taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the n gene, open reading frame (orf) 3, and orf 8. the overall linear range of this assay was from at least 10(1) to 10(6) copies per reaction, and the detection limit could reach less than 10 copies per reaction. the quantification results for sars-cov from cell culture correlated well with those of the rt-pcr by using any two of the three sets of primer and probe used in this assay. however, the results of quantification of sars-cov obtained by using a few available throat swab specimens from sars patients and the n gene as the target were almost 10 times higher than those obtained by using orf 3 and orf 8. using this assay, we also detected an apparently sars-cov-related coronavirus in the throat swab specimens from masked palm civets in the west part of hubei province, people's republic of china.
WOS关键词	SARS-ASSOCIATED CORONAVIRUS ; POLYMERASE-CHAIN-REACTION ; DNA PROBES ; SEQUENCE ; CHINA
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000229090100001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375205
专题	武汉病毒研究所
通讯作者	Wang, HZ
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430072, Peoples R China 2.Hubei Ctr Dis Control & Prevent, Wuhan, Hubei, Peoples R China 3.Wuhan Ctr Dis Control & Prevent, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Hu, WQ,Bai, BK,Hu, ZH,et al. Development and evaluation of a multitarget real-time taqman reverse transcription-pcr assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets[J]. Journal of clinical microbiology,2005,43(5):2041-2046.
APA	Hu, WQ.,Bai, BK.,Hu, ZH.,Chen, Z.,An, XF.,...&Wang, HZ.(2005).Development and evaluation of a multitarget real-time taqman reverse transcription-pcr assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets.Journal of clinical microbiology,43(5),2041-2046.
MLA	Hu, WQ,et al."Development and evaluation of a multitarget real-time taqman reverse transcription-pcr assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets".Journal of clinical microbiology 43.5(2005):2041-2046.

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