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	A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins
	Chen, Chaohui1; Liu, Yufei1; Zheng, Zhenhua2; Zhou, Guohua1; Ji, Xinghu1; Wang, Hanzhong2; He, Zhike1
刊名	Analytica chimica acta
	2015-06-23
卷号	880页码:1-7
关键词	Assembly Colorimetric Amplification Protein detection
ISSN号	0003-2670
DOI	10.1016/j.aca.2015.05.010
通讯作者	He, zhike(zhkhe@whu.edu.cn)
英文摘要	An amplified colorimetric method has been developed for the detection of protein and cancer cells based on the assembly of nucleic acids and proteins for the first time. in this process, the assembly of nucleic acids was triggered by a biotinylated dna strand after a sandwich immunoreaction. the biotinylated dna strand and sandwich immunocomplex were connected by streptavidin. then, the assembly of biotinylated bovine serum albumin (biotin-bsa) and streptavidin-horseradish peroxidase (sa-hrp) occurred at a node of the assembled products of nucleic acids through the biotin-streptavidin reaction. under the catalysis of horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine (tmb) was oxidized by h2o2 and the oxidized product was analyzed by its uv-vis absorbance signal and sensitive colorimetric detection. this colorimetric sensor could not only achieve the quantitative determination of protein by uv-vis absorbance but could also be applied for semiquantitative determination by digital visualization. using alpha-fetoprotein (afp) as the model target, this proposed colorimetric method showed a wide linear range from 5 pg/ml to 1 ng/ml with a detection limit of 1.95 pg/ml by the instrument, and even 5 pg/ml target protein could be distinguished simply by the naked eye. this approach was then expanded to detect cancer cells based on the recognition of folic acid receptors that were over-expressed on the cancer cells by folic acid-tethered dna. more importantly, this strategy can be further used as a universal colorimetric method for the determination of viruses or other proteins by changing the corresponding antibodies. (c) 2015 elsevier b.v. all rights reserved.
WOS关键词	HYBRIDIZATION CHAIN-REACTION ; ROLLING CIRCLE AMPLIFICATION ; ELECTROCHEMICAL PROXIMITY ASSAY ; SIGNAL AMPLIFICATION ; DNA ; NANOPARTICLES ; COMPLEX ; STRATEGY ; APTAMERS ; ENZYME
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000356234800001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375172
专题	武汉病毒研究所
通讯作者	He, Zhike
作者单位	1.Wuhan Univ, Coll Chem & Mol Sci, Key Lab Analyt Chem Biol & Med, Minist Educ, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Chen, Chaohui,Liu, Yufei,Zheng, Zhenhua,et al. A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins[J]. Analytica chimica acta,2015,880:1-7.
APA	Chen, Chaohui.,Liu, Yufei.,Zheng, Zhenhua.,Zhou, Guohua.,Ji, Xinghu.,...&He, Zhike.(2015).A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins.Analytica chimica acta,880,1-7.
MLA	Chen, Chaohui,et al."A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins".Analytica chimica acta 880(2015):1-7.

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