| Mesenchymal stem cells enhance the metastasis of 3d-cultured hepatocellular carcinoma cells | |
| Liu, Chang1,2; Liu, Yang1; Xu, Xiao-xi1,2; Guo, Xin1; Sun, Guang-wei1; Ma, Xiao-jun1 | |
| 刊名 | Bmc cancer
 |
| 2016-07-30 | |
| 卷号 | 16页码:9 |
| 关键词 | Three-dimensional cell culture Umbilical cord mensenchymal stem cells Hepatocellular carcinoma Metastasis Tgf-beta |
| ISSN号 | 1471-2407 |
| DOI | 10.1186/s12885-016-2595-4 |
| 通讯作者 | Sun, guang-wei(sungw@dicp.ac.cn) |
| 英文摘要 | Background: accumulating evidences have demonstrated that mesenchymal stem cells (msc) could be recruited to the tumor microenvironment. umbilical cord mesenchymal stem cells (ucmsc) were attractive vehicles for delivering therapeutic agents against cancer. nevertheless, the safety of ucmsc in the treatment of tumors including hepatocellular carcinoma (hcc) was still undetermined. methods: in this study, an in vitro co-culture system was established to evaluate the effect of ucmsc on the cell growth, cancer stem cell (csc) characteristics, drug resistance, metastasis of 3d-cultured hcc cells, and the underlying mechanism was also investigated. results: it was found that after co-cultured with ucmsc, the metastatic ability of 3d-cultured hcc cells was significantly enhanced as indicated by up-regulation of matrix metalloproteinase (mmp), epithelial-mesenchymal transition (emt)-related genes, and migration ability. however, cell growth, drug resistance and csc-related gene expression of hcc cells were not affected by ucmsc. moreover, emt was reversed, mmp-2 expression was down-regulated, and migration ability of hcc cell was significantly inhibited when tgf-beta receptor inhibitor sb431542 was added into the co-culture system. conclusions: therefore, these data indicated that ucmsc could significantly enhance the tumor cell metastasis, which was due to the emt of hcc cells induced by tgf-beta. |
| WOS关键词 | HUMAN UMBILICAL-CORD ; IN-VITRO ; TUMOR PROGRESSION ; CANCER CELLS ; GROWTH ; TRANSITION ; PROMOTE ; MODEL ; TARGET ; GENE |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| 出版者 | BIOMED CENTRAL LTD |
| WOS记录号 | WOS:000381216000002 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375156 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Guang-wei |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chang,Liu, Yang,Xu, Xiao-xi,et al. Mesenchymal stem cells enhance the metastasis of 3d-cultured hepatocellular carcinoma cells[J]. Bmc cancer,2016,16:9. |
| APA | Liu, Chang,Liu, Yang,Xu, Xiao-xi,Guo, Xin,Sun, Guang-wei,&Ma, Xiao-jun.(2016).Mesenchymal stem cells enhance the metastasis of 3d-cultured hepatocellular carcinoma cells.Bmc cancer,16,9. |
| MLA | Liu, Chang,et al."Mesenchymal stem cells enhance the metastasis of 3d-cultured hepatocellular carcinoma cells".Bmc cancer 16(2016):9. |
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