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	Three-fragment fluorescence complementation coupled with photoactivated localization microscopy for nanoscale imaging of ternary complexes
	Chen, Minghai1,3; Liu, Sanying1,3; Li, Wei1; Zhang, Zhiping1; Zhang, Xiaowei1; Zhang, Xian-En2; Cui, Zongqiang1
刊名	Acs nano
	2016-09-01
卷号	10期号:9页码:8482-8490
关键词	Mirisfp Tffc Palm Ternary complexes G protein
ISSN号	1936-0851
DOI	10.1021/acsnano.6b03543
通讯作者	Zhang, xian-en(zhangxe@ibp.ac.cn) ; Cui, zongqiang(czq@wh.iov.cn)
英文摘要	Many cellular processes are governed by molecular machineries that involve multiple protein interactions. however, visualizing and identifying multiprotein complexes such as ternary complexes inside cells is always challenging, particularly in the subdiffraction cellular space. here, we developed a three-fragment fluorescence complementation system (tffc) based on the splitting of a photoactivatable fluorescent protein, mirisfp, for the imaging of ternary complexes inside living cells. using a combination of tffc and photoactivated localization microscopy (palm), namely, the tffc-palm technique, we are able to identify the multi-interaction of a ternary complex with nanometer-level spatial resolution and single-molecule sensitivity. the tffc-palm system has been further applied to the analysis of the g(s) ternary complex, which is composed of alpha(s), beta(1), and gamma(2) subunits, providing further insights into the subcellular localization and function of g protein subunits at the single-molecule level. the tffc-palm represents a valuable method for the visualization and identification of ternary complexes inside cells at the nanometer scale.
WOS关键词	BETA-GAMMA-SUBUNITS ; PROTEIN-PROTEIN INTERACTIONS ; ENERGY-TRANSFER BRET ; LIVING CELLS ; FRET MICROSCOPY ; PLASMA-MEMBRANE ; VISUALIZATION ; SUPERRESOLUTION ; G-ALPHA(S) ; MECHANISM
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000384399300040
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375027
专题	中国科学院大学
通讯作者	Zhang, Xian-En; Cui, Zongqiang
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Minghai,Liu, Sanying,Li, Wei,et al. Three-fragment fluorescence complementation coupled with photoactivated localization microscopy for nanoscale imaging of ternary complexes[J]. Acs nano,2016,10(9):8482-8490.
APA	Chen, Minghai.,Liu, Sanying.,Li, Wei.,Zhang, Zhiping.,Zhang, Xiaowei.,...&Cui, Zongqiang.(2016).Three-fragment fluorescence complementation coupled with photoactivated localization microscopy for nanoscale imaging of ternary complexes.Acs nano,10(9),8482-8490.
MLA	Chen, Minghai,et al."Three-fragment fluorescence complementation coupled with photoactivated localization microscopy for nanoscale imaging of ternary complexes".Acs nano 10.9(2016):8482-8490.

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