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	Determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive ga14/uas system in aedes aegypti mosquitoes
	Zhao, Bo1; Hou, Yuan2,3; Wang, Jianjun1,4; Kokoza, Vladimir A.1; Saha, Tusar T.1; Wang, Xue-Li2,3; Lin, Ling1; Zou, Zhen2,3; Raikhel, Alexander S.1,5
刊名	Insect biochemistry and molecular biology
	2016-10-01
卷号	77页码:69-77
关键词	Juvenile hormone Early trypsin Methoprene-tolerant Mosquito Hormone action Transgenesis
ISSN号	0965-1748
DOI	10.1016/j.ibmb.2016.08.003
通讯作者	Zou, zhen(zouzhen@ioz.ac.cn) ; Raikhel, alexander s.(alexander.raikhel@ucr.edu)
英文摘要	In anautogenous mosquitoes, juvenile hormone iii (jh) plays an essential role in female post-eclosion (pe) development, preparing them for subsequent blood feeding and egg growth. we re-examined the jh titer during the reproductive cycle of female aedes aegypti mosquitoes. using liquid chromatography coupled with triple tandem mass spectrometry (lc-ms/ms/ms), we have shown that it reaches its peak at 48-54 h pe in the female hemolymph and at 72 h pe in whole body extracts. this method represents an effective assay for determination of jh titers. the 2.1-kb 5' promoter region of the early trypsin (et) gene, which is specifically expressed in the female midgut under the control of jh during the pe phase, was utilized to genetically engineer the ae. aegypti mosquito line with the et-gal4 activator. we then established the et-gal4 > uas-enhanced green fluorescent protein (egfp) system in ae. aegypti. in et-gal4 > uas-egfp female mosquitoes, the intensity of the midgut-specific egfp signal was observed to correspond to the et gene transcript level and follow the jh titer during the pe phase. the egfp signal and the egfp transcript level were significantly diminished in midguts of transgenic female mosquitoes after rna interference depletion of the jh receptor methoprene-tolerant (met), providing evidence of the control of et gene expression by met. topical jh application caused premature enhancement of the egfp signal and the egfp transcript level in midguts of newly eclosed et-gal4 > uas-egfp female mosquitoes, in which endogenous jh titer is still low. hence, this novel et-gal4 > uas system permits jh-dependent gene overexpression in the midgut of ae. aegypti female mosquitoes prior to a blood meal. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词	EARLY TRYPSIN ; METHOPRENE-TOLERANT ; GENE-EXPRESSION ; MASS-SPECTROMETRY ; YELLOW-FEVER ; ZIKA VIRUS ; MIDGUT ; RECEPTOR ; ACID ; HETERODIMER
WOS研究方向	Biochemistry & Molecular Biology ; Entomology
WOS类目	Biochemistry & Molecular Biology ; Entomology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000385321700007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374827
专题	中国科学院大学
通讯作者	Zou, Zhen; Raikhel, Alexander S.
作者单位	1.Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA 2.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China 5.Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA
推荐引用方式 GB/T 7714	Zhao, Bo,Hou, Yuan,Wang, Jianjun,et al. Determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive ga14/uas system in aedes aegypti mosquitoes[J]. Insect biochemistry and molecular biology,2016,77:69-77.
APA	Zhao, Bo.,Hou, Yuan.,Wang, Jianjun.,Kokoza, Vladimir A..,Saha, Tusar T..,...&Raikhel, Alexander S..(2016).Determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive ga14/uas system in aedes aegypti mosquitoes.Insect biochemistry and molecular biology,77,69-77.
MLA	Zhao, Bo,et al."Determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive ga14/uas system in aedes aegypti mosquitoes".Insect biochemistry and molecular biology 77(2016):69-77.

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