Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis

	Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis
	Gao, Peng 2; Lu, Chen 1; Zhang, Fengxia 2,4; Sang, Ping 2,3; Yang, Dawei 2,3; Li, Xiang 2; Kong, Hongwei 2; Yin, Peiyuan 2; Tian, Jing 2,3; Lu, Xin 2
刊名	analyst
	2008-09-01
卷号	133 期号:9 页码:1214-1220
通讯作者	许国旺
产权排序	1;1
英文摘要	ankylosing spondylitis (as) is a chronic inflammatory arthritis that predominantly affects the axial skeleton in adolescent patients. the natural history of the disease remains poorly characterized. in this study, we combined gc-ms and lc-ms techniques to evaluate the major metabolic changes in the plasma of as patients in view of metabonomics. univariate and multivariate analysis were employed for altered metabolite comparison and pattern recognition. application of supervised partial least-squares discrminant analysis to either gc-ms or lc-ms data allowed accurate discrimination of as patients from normal controls, demonstrating its potential diagnostic utilization. in addition, as patients presented elevated plasma concentrations of proline, glucose, phosphate, urea, glycerol, phenylalanine and homocysteine but reduced levels of phosphocholines, tryptophan and a bipeptide - phenylalanyl-phenylalanine. in the context of their involved metabolic pathways, the identified metabolites were discussed accordingly. this investigation primarily proved that integrated chromatography-mass spectrometry and integrated uni- and multi-variate statistical analysis facilitated metabonomics to be a more promising tool in disease research.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	chromatography-mass spectrometry ; research-and-development ; bone-mineral density ; systems biology ; metabolic interactions ; rheumatoid-arthritis ; biochemical markers ; endothelial-cells ; gene-expression ; cultured human
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000259449400014
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/100453]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.Dalian Polytech Univ, Dept Modern Technol, Dalian 116034, Peoples R China 4.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Gao, Peng,Lu, Chen,Zhang, Fengxia,et al. Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis[J]. analyst,2008,133(9):1214-1220.
APA	Gao, Peng.,Lu, Chen.,Zhang, Fengxia.,Sang, Ping.,Yang, Dawei.,...&Xu, Guowang.(2008).Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis.analyst,133(9),1214-1220.
MLA	Gao, Peng,et al."Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis".analyst 133.9(2008):1214-1220.