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	Quantum dot-fluorescence in situ hybridisation for ectromelia virus detection based on biotin-streptavidin interactions
	Wang, Ting1,3; Zheng, Zhenhua1; Zhang, Xian-En2; Wang, Hanzhong1
刊名	Talanta
	2016-09-01
卷号	158页码:179-184
关键词	Quantum-dot Fluorescence in situ hybridisation Ectromelia virus Virus dna detection
ISSN号	0039-9140
DOI	10.1016/j.talanta.2016.04.052
通讯作者	Zhang, xian-en(zhangxe@ibp.ac.cn) ; Wang, hanzhong(wanghz@wh.iov.cn)
英文摘要	Ectromelia virus (ectv) is an pathogen that can lead to a lethal, acute toxic disease known as mousepox in mice. prevention and control of ectv infection requires the establishment of a rapid and sensitive diagnostic system for detecting the virus. in the present study, we developed a method of quantum-dot fluorescence based in situ hybridisation for detecting ectv genome dna. using biotin-dutp to replace dttp, biotin was incorporated into a dna probe during polymerase chain reaction. high sensitivity and specificity of ectv dna detection were displayed by fluorescent quantum dots based on biotin-streptavidin interactions. ecfv dna was then detected by streptavidin-conjugated quantum dots that bound the biotin-labelled probe. results indicated that the established method can visualise ectv genomic dna in both infected cells and mouse tissues. to our knowledge, this is the first study reporting quantum-dot fluorescence based in situ hybridisation for the detection of viral nucleic acids, providing a reference for the identification and detection of other viruses. (c) 2016 published by elsevier b.v.
WOS关键词	CAUSATIVE AGENT ; MOUSEPOX ; NANOCRYSTALS ; INFECTION ; ANTIBODY
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000379370400023
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374715
专题	中国科学院大学
通讯作者	Zhang, Xian-En; Wang, Hanzhong
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ting,Zheng, Zhenhua,Zhang, Xian-En,et al. Quantum dot-fluorescence in situ hybridisation for ectromelia virus detection based on biotin-streptavidin interactions[J]. Talanta,2016,158:179-184.
APA	Wang, Ting,Zheng, Zhenhua,Zhang, Xian-En,&Wang, Hanzhong.(2016).Quantum dot-fluorescence in situ hybridisation for ectromelia virus detection based on biotin-streptavidin interactions.Talanta,158,179-184.
MLA	Wang, Ting,et al."Quantum dot-fluorescence in situ hybridisation for ectromelia virus detection based on biotin-streptavidin interactions".Talanta 158(2016):179-184.

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