Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography-mass spectrometry

	Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography-mass spectrometry
	Ma, Chenfei 1; Wang, Huahong 2; Lu, Xin 1; Xu, Guowang 1; Liu, Benye 2
刊名	journal of chromatography a
	2008-04-04
卷号	1186 期号:1-2 页码:412-419
关键词	Artemisia annua L. metabolic fingerprinting metabolomics pattern recognition developmental stage
通讯作者	许国旺
产权排序	1;1
英文摘要	artemisia annua l. is an annual herb native of asia and this plant has been famous for the discovery of the anti-malarial drug artemisinin since 1971. in this work, to investigate variety of whole metabolites, metabolic fingerprinting analysis of a. annua l. was carried out by gc and gc-ms coupled with trimethylsilyl derivatisation. principal component analysis and partial least squares discriminant analysis were employed to classify gc data of a. annua l. samples at five developmental stages. the results indicated that there was no distinct difference of metabolites between control (001) and transgenic strain (f4) from the tender seedling stage to adult seedling stage, but clear differences were detected at pre-flower budding stage, flower budding stage and full flowering stage. three precursors of artemisinin biosynthesis were studied at five developmental stages and found that a possible bottleneck exists in the conversion from artemisinic acid or dihydroartemisinic acid to artemisinin. (c) 2007 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; dihydroartemisinic acid ; biosynthetic precursors ; identification ; h-1-nmr ; plants
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000254884700036
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/100431]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ma, Chenfei,Wang, Huahong,Lu, Xin,et al. Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography-mass spectrometry[J]. journal of chromatography a,2008,1186(1-2):412-419.
APA	Ma, Chenfei,Wang, Huahong,Lu, Xin,Xu, Guowang,&Liu, Benye.(2008).Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography-mass spectrometry.journal of chromatography a,1186(1-2),412-419.
MLA	Ma, Chenfei,et al."Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography-mass spectrometry".journal of chromatography a 1186.1-2(2008):412-419.