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	Selectively improving nikkomycin z production by blocking the imidazolone biosynthetic pathway of nikkomycin x and uracil feeding in streptomyces ansochromogenes
	Liao,Guojian1,2; Li,Jine1,2; Li,Lei1; Yang,Haihua1; Tian,Yuqing1; Tan,Huarong1
刊名	Microbial cell factories
	2009-11-23
卷号	8期号:1
ISSN号	1475-2859
DOI	10.1186/1475-2859-8-61
通讯作者	Tan,huarong(tanhr@sun.im.ac.cn)
英文摘要	Abstractbackgroundnikkomycins are a group of peptidyl nucleoside antibiotics and act as potent inhibitors of chitin synthases in fungi and insects. nikkomycin x and z are the main components produced by streptomyces ansochromogenes. of them, nikkomycin z is a promising antifungal agent with clinical significance. since highly structural similarities between nikkomycin z and x, separation of nikkomycin z from the culture medium of s. ansochromogenes is difficult. thus, generating a nikkomycin z selectively producing strain is vital to scale up the nikkomycin z yields for clinical trials.resultsa nikkomycin z producing strain (sanpdm) was constructed by blocking the imidazolone biosynthetic pathway of nikkomycin x via genetic manipulation and yielded 300 mg/l nikkomycin z and abolished the nikkomycin x production. to further increase the yield of nikkomycin z, the effects of different precursors on its production were investigated. precursors of nucleoside moiety (uracil or uridine) had a stimulatory effect on nikkomycin z production while precursors of peptidyl moiety (l-lysine and l-glutamate) had no effect. sanpdm produced the maximum yields of nikkomycin z (800 mg/l) in the presence of uracil at the concentration of 2 g/l and it was approximately 2.6-fold higher than that of the parent strain.conclusiona high nikkomycin z selectively producing was obtained by genetic manipulation combined with precursors feeding. the strategy presented here might be applicable in other bacteria to selectively produce targeted antibiotics.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/1475-2859-8-61
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374323
专题	中国科学院大学
通讯作者	Tan,Huarong
作者单位	1.State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences 2.Graduate School of Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Liao,Guojian,Li,Jine,Li,Lei,et al. Selectively improving nikkomycin z production by blocking the imidazolone biosynthetic pathway of nikkomycin x and uracil feeding in streptomyces ansochromogenes[J]. Microbial cell factories,2009,8(1).
APA	Liao,Guojian,Li,Jine,Li,Lei,Yang,Haihua,Tian,Yuqing,&Tan,Huarong.(2009).Selectively improving nikkomycin z production by blocking the imidazolone biosynthetic pathway of nikkomycin x and uracil feeding in streptomyces ansochromogenes.Microbial cell factories,8(1).
MLA	Liao,Guojian,et al."Selectively improving nikkomycin z production by blocking the imidazolone biosynthetic pathway of nikkomycin x and uracil feeding in streptomyces ansochromogenes".Microbial cell factories 8.1(2009).

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