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	Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach
	Xu, W; Zhang, X; Lou, SQ; Wang, KB; Huang, YG; Kuang, TY
刊名	Science in china series c-life sciences
	1998-06-01
卷号	41期号:3页码:265-271
关键词	Light-harvesting chlorophyll a/b protein complex Two-dimensional crystallization Electron microscopy Image processing Structure analysis Cucumber Spinach
ISSN号	1006-9305
通讯作者	Xu, w()
英文摘要	Large and well-ordered two-dimensional (2d) crystals of the light-harvesting chlorophyll a/b protein complexes (lhc-ii) from cucumber and spinach chloroplasts were produced by the so-called batch method. the two-dimensional structures of these crystals were examined at about 1.5 nm resolution by electron microscopy and image processing. the projection maps showed that there were similar, but not identical, structure features between two different lhc-ii complexes. a comparison between 2d crystal formations of the two different lhc-ii complexes was done and some factors affecting 2d crystallization of the membrane proteins were analyzed. the relations of the structures of the lhc-ii complexes to their polypeptide components and chi a/b ratio were also discussed.
WOS关键词	CHLOROPHYLL-A/B-PROTEIN ; LIGHT-HARVESTING COMPLEX ; 3-DIMENSIONAL STRUCTURE ; ELECTRON CRYSTALLOGRAPHY ; CRYSTALS ; MICROSCOPY ; MODEL
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000074448600007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373824
专题	物理研究所
通讯作者	Xu, W
作者单位	1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Beijing Lab Electron Microscopy, Ctr Condensed Matter Phys, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xu, W,Zhang, X,Lou, SQ,et al. Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach[J]. Science in china series c-life sciences,1998,41(3):265-271.
APA	Xu, W,Zhang, X,Lou, SQ,Wang, KB,Huang, YG,&Kuang, TY.(1998).Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach.Science in china series c-life sciences,41(3),265-271.
MLA	Xu, W,et al."Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach".Science in china series c-life sciences 41.3(1998):265-271.

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