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	Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression
	Hao, Sujuan1,2; Zhang, Junmei1,2; Chen, Zhen1; Xu, Huanzhou1,2; Wang, Hanzhong1; Guan, Wuxiang1,3
刊名	Journal of virology
	2017-02-01
卷号	91期号:3页码:16
关键词	Hbov Alternative polyadenylation Capsid protein expression Parvovirus
ISSN号	0022-538X
DOI	10.1128/jvi.02026-16
通讯作者	Guan, wuxiang(guanwx@wh.iov.cn)
英文摘要	Alternative processing of human bocavirus (hbov) p5 promoter-transcribed rna is critical for generating the structural and nonstructural protein-encoding mrna transcripts. the regulatory mechanism by which hbov rna transcripts are polyadenylated at proximal [(pa)p] or distal [(pa)d] polyadenylation sites is still unclear. we constructed a recombinant hbov infectious clone to study the alternative polyadenylation regulation of hbov. surprisingly, in addition to the reported distal polyadenylation site, (pa)d, a novel distal polyadenylation site, (pa)d2, which is located in the right-end hairpin (reh), was identified during infectious clone transfection or recombinant virus infection. (pa)d2 does not contain typical hexanucleotide polyadenylation signal, upstream elements (use), or downstream elements (dse) according to sequence analysis. further study showed that hbov nonstructural protein ns1, reh, and cis elements of (pa) d were necessary and sufficient for efficient polyadenylation at (pa)d2. the distance and sequences between (pa)d and (pa)d2 also played a key role in the regulation of polyadenylation at (pa)d2. finally, we demonstrated that efficient polyadenylation at (pa)d2 resulted in increased hbov capsid mrna transcripts and protein translation. thus, our study revealed that all the bocaviruses have distal poly(a) signals on the right-end palindromic terminus, and alternative polyadenylation at the hbov 3' end regulates its capsid expression. importance the distal polyadenylation site, (pa)d, of hbov is located about 400 nucleotides (nt) from the right-end palindromic terminus, which is different from those of bovine parvovirus (bpv) and canine minute virus (mvc) in the same genus whose distal polyadenylation is located in the right-end stem-loop structure. a novel polyadenylation site, (pa)d2, was identified in the right-end hairpin of hbov during infectious clone transfection or recombinant virus infection. sequence analysis showed that (pa)d2 does not contain typical polyadenylation signals, and the last 42 nt form a stem-loop structure which is almost identical to that of mvc. further study showed that ns1, reh, and cis elements of (pa) d are required for efficient polyadenylation at (pa)d2. polyadenylation at (pa)d2 enhances capsid expression. our study demonstrates alternative polyadenylation at the 3' end of hbov and suggests an additional mechanism by which capsid expression is regulated.
WOS关键词	RESPIRATORY-TRACT INFECTIONS ; PRECURSOR MESSENGER-RNA ; HUMAN PARVOVIRUS B19 ; INTERNAL POLYADENYLATION ; ACUTE GASTROENTERITIS ; MINUTE VIRUS ; NONSTRUCTURAL PROTEINS ; CHILDREN ; GENOME ; CELLS
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000393194600026
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373470
专题	武汉病毒研究所
通讯作者	Guan, Wuxiang
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Hubei Collaborat Innovat Ctr Ind Fermentat, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Hao, Sujuan,Zhang, Junmei,Chen, Zhen,et al. Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression[J]. Journal of virology,2017,91(3):16.
APA	Hao, Sujuan,Zhang, Junmei,Chen, Zhen,Xu, Huanzhou,Wang, Hanzhong,&Guan, Wuxiang.(2017).Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression.Journal of virology,91(3),16.
MLA	Hao, Sujuan,et al."Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression".Journal of virology 91.3(2017):16.

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