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	Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in china
	Wang, Bo1,2; Yang, Xing-Lou1,2; Li, Wen1,2; Zhu, Yan1,2; Ge, Xing-Yi1,2; Zhang, Li-Biao3; Zhang, Yun-Zhi4; Bock, Claus-Thomas5; Shi, Zheng-Li1,2
刊名	Virology journal
	2017-02-22
卷号	14页码:10
关键词	Bat Natural reservoir Hepadnavirus Hepevirus Genome characterization
ISSN号	1743-422X
DOI	10.1186/s12985-017-0706-8
通讯作者	Shi, zheng-li(zlshi@wh.iov.cn)
英文摘要	Background: in recent years, novel hepadnaviruses, hepeviruses, hepatoviruses, and hepaciviruses have been discovered in various species of bat around the world, indicating that bats may act as natural reservoirs for these hepatitis viruses. in order to further assess the distribution of hepatitis viruses in bat populations in china, we tested the presence of these hepatitis viruses in our archived bat liver samples that originated from several bat species and various geographical regions in china. methods: a total of 78 bat liver samples (involving two families, five genera, and 17 species of bat) were examined using nested or heminested reverse transcription pcr (rt-pcr) with degenerate primers. full-length genomic sequences of two virus strains were sequenced followed by phylogenetic analyses. results: four samples were positive for hepadnavirus, only one was positive for hepevirus, and none of the samples were positive for hepatovirus or hepacivirus. the hepadnaviruses were discovered in the horseshoe bats, rhinolophus sinicus and rhinolophus affinis, and the hepevirus was found in the whiskered bat myotis davidii. the full-length genomic sequences were determined for one of the two hepadnaviruses identified in r.sinicus (designated bthbvrs3364) and the hepevirus (designated bthevmd2350). a sequence identity analysis indicated that bthbvrs3364 had the highest degree of identity with a previously reported hepadnavirus from the roundleaf bat, hipposideros pomona, from china, and bthevmd2350 had the highest degree of identity with a hepevirus found in the serotine bat, eptesicus serotinus, from germany, but it exhibited high levels of divergence at both the nucleotide and the amino acid levels. conclusions: this is the first study to report that the chinese horseshoe bat and the chinese whiskered bat have been found to carry novel hepadnaviruses and a novel hepevirus, respectively. the discovery of bthbvrs3364 further supports the significance of host switches evolution while opposing the co-evolutionary theory associated with hepadnaviruses. according to the latest criterion of the international committee on taxonomy of viruses (ictv), we hypothesize that bthevmd2350 represents an independent genotype within the species orthohepevirus d of the family hepeviridae.
WOS关键词	EMERGING INFECTIOUS-DISEASES ; VIRUSES ; RESERVOIR ; ORIGINS
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000395991100002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373463
专题	武汉病毒研究所
通讯作者	Shi, Zheng-Li
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Wuhan, Peoples R China 3.Guangdong Inst Appl Biol Resource, Guangzhou 510260, Guangdong, Peoples R China 4.Yunnan Inst Endem Dis Control & Prevent, Lab Zoonosis Control & Prevent, Dali 671000, Peoples R China 5.Robert Koch Inst, Dept Infect Dis, Berlin, Germany
推荐引用方式 GB/T 7714	Wang, Bo,Yang, Xing-Lou,Li, Wen,et al. Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in china[J]. Virology journal,2017,14:10.
APA	Wang, Bo.,Yang, Xing-Lou.,Li, Wen.,Zhu, Yan.,Ge, Xing-Yi.,...&Shi, Zheng-Li.(2017).Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in china.Virology journal,14,10.
MLA	Wang, Bo,et al."Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in china".Virology journal 14(2017):10.

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