QQ客服 官方微博 反馈留言

	Mutations in the c-terminus of hbov ns1 affect the function of np1
	Zhang, Junmei1,2; Bai, Yan3; Zhu, Bing4; Hao, Sujuan1,2; Chen, Zhen1; Wang, Hanzhong1; Guan, Wuxiang1
刊名	Scientific reports
	2017-08-07
卷号	7页码:12
ISSN号	2045-2322
DOI	10.1038/s41598-017-06513-4
通讯作者	Guan, wuxiang(guanwx@wh.iov.cn)
英文摘要	Human bocavirus 1 (hbov1) is an autonomous parvovirus in the bocaparvovirus genus. the multifunctional nuclear protein np1 is involved in viral replication. in the present study, we found that the mutations in the c-terminus of ns1 affected np1 function in viral replication. knocking out np1 expression in the recombinant infectious clone, on which the c-terminus of ns1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. the result suggested that np1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where np1 is essential for viral replication. further studies showed that clinical mutations in the np1 region did not affect viral genome replication, and up1 promoted viral dna replication. our results suggested that the c-terminus of ns1 is important for viral replication and may be a target for regulating the replication of the viral genome.
WOS关键词	HUMAN BOCAVIRUS 1 ; MINUTE VIRUS ; NONSTRUCTURAL PROTEINS ; HOSPITALIZED CHILDREN ; RESPIRATORY VIRUSES ; HUMAN PARVOVIRUS ; DNA-REPLICATION ; ORIGIN ; INFECTION ; HAIRPIN
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000407080100037
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373366
专题	武汉病毒研究所
通讯作者	Guan, Wuxiang
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Huazhong Univ Sci & Technol, Pediat Dept, Tongji Med Coll, Union Hosp, Wuhan, Hubei, Peoples R China 4.Guangzhou Women & Childrens Med Ctr, Ctr Lab, Guangzhou 510120, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Junmei,Bai, Yan,Zhu, Bing,et al. Mutations in the c-terminus of hbov ns1 affect the function of np1[J]. Scientific reports,2017,7:12.
APA	Zhang, Junmei.,Bai, Yan.,Zhu, Bing.,Hao, Sujuan.,Chen, Zhen.,...&Guan, Wuxiang.(2017).Mutations in the c-terminus of hbov ns1 affect the function of np1.Scientific reports,7,12.
MLA	Zhang, Junmei,et al."Mutations in the c-terminus of hbov ns1 affect the function of np1".Scientific reports 7(2017):12.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。