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	Development and evaluation of one-step multiplex real-time rt-pcr assay for simultaneous detection of zika virus and chikungunya virus
	Liu, Si-Qing1; Li, Xiao1,2; Deng, Cheng-Lin1; Yuan, Zhi-Ming1; Zhang, Bo1
刊名	Journal of medical virology
	2018-03-01
卷号	90期号:3页码:389-396
关键词	Amplification Chikungunya virus Multiplex real-time rt-pcr assay Zika virus
ISSN号	0146-6615
DOI	10.1002/jmv.24970
通讯作者	Zhang, bo(zhangbo@wh.iov.cn)
英文摘要	Zika virus (zikv) and chikungunya virus (chikv) are important human pathogens and mosquito-borne arboviruses, which have resembling history, common vectors, circulating regions, and indistinguishable clinical symptoms. wide geographical range that is suitable for zikv and chikv transmission underlines the concern about the impact of epidemic and endemic infections on burden of public health. in the present study, a highly sensitive and specific one-step multiplex real-time rt-pcr assay was developed and evaluated for simultaneous detection and quantification of zikv and chikv. the single reaction assay employs two pairs of primers and two taqman probes that differentiate zikv and chikv infections. the entire viral genomic rna in vitro transcribed from full-length infectious clones were used to generate the standard curves for absolute quantification in subsequent tests. the detection limit of the one-step multiplex assay was 1 and 0.5 pfu for infectious zikv and chikv, respectively. the assessment of specificity indicated this assay is highly specific to targeted viruses showing no amplification of a variety of other flaviviruses. our assay was able to detect geographically separated and phylogenetically diverse strains of zikv and chikv. on the applicability of monitoring viral multiplication in cells and testing clinical samples, the one-step multiplex assay provided efficient and accurate determination. the one-step multiplex real-time rt-pcr assay offers a valuable tool for detection of zikv and chikv and potentially contributes to general surveillance and clinical treatment.
WOS关键词	INFECTIOUS CDNA-CLONE ; ENCEPHALITIS-VIRUS ; DENGUE VIRUSES ; FEVER VIRUS ; TRANSMISSION ; MUTATIONS ; INSIGHTS ; REVEALS ; CHINA
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	WILEY
WOS记录号	WOS:000419510600001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373350
专题	武汉病毒研究所
通讯作者	Zhang, Bo
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Si-Qing,Li, Xiao,Deng, Cheng-Lin,et al. Development and evaluation of one-step multiplex real-time rt-pcr assay for simultaneous detection of zika virus and chikungunya virus[J]. Journal of medical virology,2018,90(3):389-396.
APA	Liu, Si-Qing,Li, Xiao,Deng, Cheng-Lin,Yuan, Zhi-Ming,&Zhang, Bo.(2018).Development and evaluation of one-step multiplex real-time rt-pcr assay for simultaneous detection of zika virus and chikungunya virus.Journal of medical virology,90(3),389-396.
MLA	Liu, Si-Qing,et al."Development and evaluation of one-step multiplex real-time rt-pcr assay for simultaneous detection of zika virus and chikungunya virus".Journal of medical virology 90.3(2018):389-396.

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