| Uncovering the rab5-independent autophagic trafficking of influenza a virus by quantum-dot-based single-virus tracking | |
| Wu, Qiu-Mei1,2; Liu, Shu-Lin1,2; Chen, Gang3,4; Zhang, Wei3,4; Sun, En-Ze1,2; Xiao, Geng-Fu5; Zhang, Zhi-Ling1,2; Pang, Dai-Wen1,2 | |
| 刊名 | Small
 |
| 2018-03-22 | |
| 卷号 | 14期号:12页码:16 |
| 关键词 | Autophagy Influenza a virus Quantum dots Rab5 Single-virus tracking |
| ISSN号 | 1613-6810 |
| DOI | 10.1002/smll.201702841 |
| 通讯作者 | Pang, dai-wen(dwpang@whu.edu.cn) |
| 英文摘要 | Autophagy is closely related to virus-induced disease and a comprehensive understanding of the autophagy-associated infection process of virus will be significant for developing more effective antiviral strategies. however, many critical issues and the underlying mechanism of autophagy in virus entry still need further investigation. here, this study unveils the involvement of autophagy in influenza a virus entry. the quantum-dot-based single-virus tracking technique assists in real-time, prolonged, and multicolor visualization of the transport process of individual viruses and provides unambiguous dissection of the autophagic trafficking of viruses. these results reveal that roughly one-fifth of viruses are ferried into cells for infection by autophagic machineries, while the remaining are not. a comprehensive overview of the endocytic- and autophagic-trafficking process indicates two distinct trafficking pathway of viruses, either dependent on rab5-positive endosomes or autophagosomes, with striking similarities. expressing dominant-negative mutant of rab5 suggests that the autophagic trafficking of viruses is independent on rab5. the present study provides dynamic, precise, and mechanistic insights into the involvement of autophagy in virus entry, which contributes to a better understanding of the relationship between autophagy and virus entry. the quantum-dot-based single-virus tracking is proven to hold promise for autophagy-related fundamental research. |
| WOS关键词 | PARTICLE TRACKING ; LIVE CELLS ; IN-VIVO ; INFECTION ; PATHWAY ; LYSOSOMES ; PROTEIN ; FUSION ; RAB7 ; REPLICATION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000428344300008 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373323 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Pang, Dai-Wen |
| 作者单位 | 1.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Coll Chem & Mol Sci,State Key Lab Virol,Inst Adv, Wuhan 430072, Hubei, Peoples R China 2.Wuhan Univ, Wuhan Inst Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Wuhan Univ, Key Lab Oral Biomed, Minist Educ, Sch & Hosp Stomatol, Wuhan 430079, Hubei, Peoples R China 4.Wuhan Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Wuhan 430079, Hubei, Peoples R China 5.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Qiu-Mei,Liu, Shu-Lin,Chen, Gang,et al. Uncovering the rab5-independent autophagic trafficking of influenza a virus by quantum-dot-based single-virus tracking[J]. Small,2018,14(12):16. |
| APA | Wu, Qiu-Mei.,Liu, Shu-Lin.,Chen, Gang.,Zhang, Wei.,Sun, En-Ze.,...&Pang, Dai-Wen.(2018).Uncovering the rab5-independent autophagic trafficking of influenza a virus by quantum-dot-based single-virus tracking.Small,14(12),16. |
| MLA | Wu, Qiu-Mei,et al."Uncovering the rab5-independent autophagic trafficking of influenza a virus by quantum-dot-based single-virus tracking".Small 14.12(2018):16. |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论