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	Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice
	Dai, Shiyu; Zhang, Tao; Zhang, Yanfang; Wang, Hualin; Deng, Fei
刊名	Virologica sinica
	2018-06-01
卷号	33期号:3页码:213-226
关键词	Zikv Baculovirus expression system Virus-like particles (vlps) Neutralizing antibodies
ISSN号	1674-0769
DOI	10.1007/s12250-018-0030-5
通讯作者	Wang, hualin(h.wang@wh.iov.cn) ; Deng, fei(df@wh.iov.cn)
英文摘要	The newly emerged mosquito-borne zika virus (zikv) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. several zikv vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, dna and rna vaccines. these have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. virus-like particles (vlps) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies. in this study, we used a new strategy to produce zikv vlps based on the baculovirus expression system and demonstrated the feasibility of their use as a vaccine candidate. the pre-membrane (prm) and envelope (e) proteins were co-expressed in insect cells and self-assembled into particles similar to zikv. we found that the zikv vlps could be quickly and easily prepared in large quantities using this system. the vlps were shown to have good immunogenicity in immunized mice, as they stimulated high levels of virus neutralizing antibody titers, zikv-specific igg titers and potent memory t cell responses. thus, the baculovirus-based zikv vlp vaccine is a safe, effective and economical vaccine candidate for use against zikv.
WOS关键词	VACCINE DEVELOPMENT ; PROTEIN ; PROTECTION ; PLATFORMS ; IMMUNITY ; SYSTEMS ; CELLS
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000436012100001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373257
专题	武汉病毒研究所
通讯作者	Wang, Hualin; Deng, Fei
作者单位	Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Dai, Shiyu,Zhang, Tao,Zhang, Yanfang,et al. Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice[J]. Virologica sinica,2018,33(3):213-226.
APA	Dai, Shiyu,Zhang, Tao,Zhang, Yanfang,Wang, Hualin,&Deng, Fei.(2018).Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice.Virologica sinica,33(3),213-226.
MLA	Dai, Shiyu,et al."Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice".Virologica sinica 33.3(2018):213-226.

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