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	Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment
	Xi, Yan1,3,4; Wang, Dianbing2; Wang, Tingting2; Huang, Lin2; Zhang, Xian-En1,2,3
刊名	Nanoscale
	2019-01-28
卷号	11期号:4页码:1737-1744
ISSN号	2040-3364
DOI	10.1039/c8nr06526h
通讯作者	Zhang, xian-en(zhangxe@ibp.ac.cn)
英文摘要	Quantitative profiling of membrane proteins on the cell surface is of great interest in tumor targeted therapy and single cell biology. however, the existing technologies are either of insufficient resolution, or unable to provide precise information on the localization of individual proteins. here, we report a new method that combines the use of quantum dot labeling, super-resolution microscopy (structured illumination microscopy, sim) and software modeling. in this proof-of-principle study, we assessed the biological effects of bestatin on individual cells from different aml cell lines expressing cd13 proteins, a potential target for tumor targeted therapy. using the proposed method, we found that the different aml cell lines exhibit different cd13 expression densities, ranging from 0.1 to 1.3 molecules per mu m(2) cell surface, respectively. importantly, bestatin treatment assays shows that its effects on cell growth inhibition, apoptosis and cell cycle change are directly proportional to the density of cd13 on the cell surface of these cell lines. the results suggest that the proposed method advances the quantitative analysis of single cell surface proteins, and that the quantitative profiling information of the target protein on single cells has potential value in targeted drug susceptibility assessment.
WOS关键词	QUANTUM DOTS ; HETEROGENEITY ; CYTOMETRY
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000459910900022
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373182
专题	武汉病毒研究所
通讯作者	Zhang, Xian-En
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Henan Univ, Coll Basic Med Sci, Kaifeng 475014, Peoples R China
推荐引用方式 GB/T 7714	Xi, Yan,Wang, Dianbing,Wang, Tingting,et al. Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment[J]. Nanoscale,2019,11(4):1737-1744.
APA	Xi, Yan,Wang, Dianbing,Wang, Tingting,Huang, Lin,&Zhang, Xian-En.(2019).Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment.Nanoscale,11(4),1737-1744.
MLA	Xi, Yan,et al."Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment".Nanoscale 11.4(2019):1737-1744.

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