QQ客服 官方微博 反馈留言

	Prevalence of wenzhu virus in small mammals in yunnan province, china
	Wang, Jinxia1,2; Yang, Xinglou3; Liu, Haizhou3; Wang, Li3; Zhou, Jihua2; Han, Xi2; Zhu, Yan3; Yang, Weihong2; Pan, Hong2; Zhang, Yunzhi1,2
刊名	Plos neglected tropical diseases
	2019-02-01
卷号	13期号:2页码:16
ISSN号	1935-2735
DOI	10.1371/journal.pntd.0007049
通讯作者	Zhang, yunzhi(zhangyunzhi1818@163.com) ; Shi, zhengli(zlshi@wh.iov.cn)
英文摘要	Background mammarenaviruses are associated with human hemorrhagic fever diseases in africa and america. recently, a rodent mammarenavirus, wenzhu virus (wenv) and related viruses, have been reported in china, cambodia, and thailand. moreover, in cambodia, these viruses were suspected to be associated with human disease. in china, yunnan province is famous for its abundant animal and plant diversity and is adjacent to several south-eastern asia countries. therefore, it is necessary to know whether wenv-related viruses, or other mammarenaviruses, are prevalent in this province. methodology/principal findings small mammals were trapped, euthanized, and sampled. mammarenavirus rna was detected using a nested reverse transcription polymerase chain reaction (rt-pcr) and quantified by real-time rt-pcr. a total of 1040 small mammals belonging to 13 genera and 26 species were trapped in yunnan province. wenv-related mammarenaviruses were detected in 41 rodent liver samples, mainly in brown rats (rattus norvegicus) and oriental house rats (r. tanezumi).viral nucleocapsid protein was detected in liver sections by indirect immunofluorescence assay. full-length-genomes were amplified by rt-pcr and used for phylogenetic analysis with the mega package. recombination analysis was performed using the simplot and recombination detection program. conclusions/significance wenv related viruses circulated in small mammals in yunnan province. whole genome sequence analysis of five selected viral strains showed that these viruses are closely related to wenvs discovered in asia and form an independent branch in the phylogenetic tree in the wenv clade. paying attention to investigate the influence of these viruses to public health is essential in the epidemic regions. author summary rodents are natural reservoirs of mammarenavirus. lymphocytic choriomeningitis virus (lcmv), isolated in asian countries during the 1990s, has a worldwide distribution and was the first mammarenavirus isolated. in 2014, a second mammarenavirus, wenzhu virus (wenv), was identified in rodents in zhejiang province of china and later in guangdong, shandong, and hainan provinces. most importantly, wenv or related viruses were reported in thailand and cambodia. in cambodia, the isolated virus was associated with human respiratory diseases. in this study, we detected wenv or related viruses in yunnan province and found a high prevalence in rats of two species (rattus norvegicus and r. tanezumi). phylogenetic analysis of the complete l and s segments of five strains showed that these viruses form an independent phylogenetic branch in wenv clade most closely related to wenvs found in china and cambodia. considering the wide spread distribution of rats and altered distribution patterns due to ecological changes, we propose that these viruses may have a wider prevalence and be found in countries from south-eastern asia to china. given that wenv may be associated with human diseases, it is necessary to improve surveillances of these viruses in their natural reservoirs and in humans.
WOS关键词	EXPERIMENTAL-INFECTION ; ETIOLOGIC AGENT ; S-RNA ; ARENAVIRUS ; MURIDAE ; RODENTS ; SHREWS ; WORLD ; LASSA ; WILD
WOS研究方向	Infectious Diseases ; Parasitology ; Tropical Medicine
WOS类目	Infectious Diseases ; Parasitology ; Tropical Medicine
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000459970700018
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373178
专题	武汉病毒研究所
通讯作者	Zhang, Yunzhi; Shi, Zhengli
作者单位	1.Dali Univ, Sch Publ Hlth, Inst Prevent Med, Dali, Peoples R China 2.Yunnan Inst Endem Dis Control & Prevent, Yunnan Prov Key Lab Zoonosis Control & Prevent, Dali, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jinxia,Yang, Xinglou,Liu, Haizhou,et al. Prevalence of wenzhu virus in small mammals in yunnan province, china[J]. Plos neglected tropical diseases,2019,13(2):16.
APA	Wang, Jinxia.,Yang, Xinglou.,Liu, Haizhou.,Wang, Li.,Zhou, Jihua.,...&Shi, Zhengli.(2019).Prevalence of wenzhu virus in small mammals in yunnan province, china.Plos neglected tropical diseases,13(2),16.
MLA	Wang, Jinxia,et al."Prevalence of wenzhu virus in small mammals in yunnan province, china".Plos neglected tropical diseases 13.2(2019):16.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。