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	Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins
	An, Jinying1; Zhai, Guijin1; Guo, Zhenchang1; Bai, Xue1; Chen, Pu1; Dong, Hanyang1; Tian, Shanshan1; Ai, Ding2; Zhang, Yukui3; Zhang, Kai1
刊名	Analytical chemistry
	2019-03-05
卷号	91期号:5页码:3221-3226
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b04781
通讯作者	Zhai, guijin(zhaiguijin@tmu.edu.cn) ; Zhang, kai(kzhang@tmu.edu.cn)
英文摘要	Phosphotyrosine (py) serves as a docking site for the recognition proteins containing py-binding (pyb) modules, such as the sh2 domain, to mediate cell signal transduction. thus, it is vital to profile these binding proteins for understanding of signal regulation. however, identification of pyb proteins remains a significant challenge due to their low abundance and typically weak and transient interactions with py sites. herein, we designed and prepared a py-peptide photoaffinity probe for the robust and specific enrichment and identification of its binding proteins. using shc1-py(317) as a paradigm, we showed that the developed probe enables to capture target protein with high selectivity and remarkable specificity even in a complex context. notably, we expanded the strategy to a combinatorial py-peptide-based photoaffinity probe by using combinatorial peptide ligand library (cpll) technique and identified 24 sh2 domain proteins, which presents a deeper profiling of pyb proteins than previous reports using affinity probes. moreover, the method can be used to mine putative pyb proteins and confirmed pkn2 as a selective binder to py, expanding the repertoire of known domain proteins. our approach provides a general strategy for rapid and robust interrogating pyb proteins and will promote the understanding of the signal transduction mechanism.
WOS关键词	TYROSINE PHOSPHORYLATION ; SH2 DOMAIN ; SPECIFICITY ; PROTEOME ; TARGET ; SITES
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000460709200009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372720
专题	大连化学物理研究所
通讯作者	Zhai, Guijin; Zhang, Kai
作者单位	1.Tianjin Med Univ, Canc Inst & Hosp,Collaborat Innovat Ctr Tianjin M, Tianjin Key Lab Med Epigenet,Key Lab Breast Canc, Key Lab Immune Microenvironm & Dis,Minist Educ,De, Tianjin 300070, Peoples R China 2.Tianjin Med Univ, Dept Physiol & Pathophysiol, Tianjin Key Lab Metab Dis, Tianjin 300070, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	An, Jinying,Zhai, Guijin,Guo, Zhenchang,et al. Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins[J]. Analytical chemistry,2019,91(5):3221-3226.
APA	An, Jinying.,Zhai, Guijin.,Guo, Zhenchang.,Bai, Xue.,Chen, Pu.,...&Zhang, Kai.(2019).Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins.Analytical chemistry,91(5),3221-3226.
MLA	An, Jinying,et al."Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins".Analytical chemistry 91.5(2019):3221-3226.

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