Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis

	Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis
	Zhou, Houjiang; Ye, Mingliang; Dong, Jing; Han, Guanghui; Jiang, Xinning; Wu, Renan; Zou, Hanfa
刊名	journal of proteome research
	2008-09-01
卷号	7 期号:9 页码:3957-3967
关键词	immobilized metal ion affinity chromatography titanium ion phosphate polymer phosphopeptide enrichment phosphoproteome analysis
ISSN号	1535-3893
通讯作者	邹汉法
产权排序	1;1
英文摘要	the elucidation of protein post-translational modifications, such as phosphorylation, remains a challenging analytical task for proteomic studies. since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically substoichiometric, a prerequisite for their identification is the specific enrichment of phosphopeptide prior to mass spectrometric analysis. here, we presented a new method termed as immobilized titanium ion affinity chromatography (ti4+-imac) for enriching phosphopeptides. a phosphate polymer, which was prepared by direct polymerization of monomers containing phosphate groups, was applied to immobilize ti4+ through the chelating interaction between phosphate groups on the polymer and ti4+. the resulting ti4+-imac resin specifically isolates phosphopeptides from a digest mixture of standard phosphoproteins and nonphosphoprotein (bsa) in a ratio as low as 1:500. ti4+-imac was further applied for phosphoproteome analysis of mouse liver. we also compared ti4+-imac to other enrichment methods including fe3+-imac, zr4+-imac, tio2 and zrcl2 and demonstrate superior selectivity and efficiency of ti4+-imac for the isolation and enrichment of phosphopeptides. the high specificity and efficiency of phosphopeptide enrichment by ti4+-imac mainly resulted from the flexibility of immobilized titanium ion with spacer arm linked to polymer beads as well as the specific interaction between immobilized titanium ion and phosphate group on phosphopeptides.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	tandem mass-spectrometry ; scale phosphorylation analysis ; highly selective enrichment ; nano-lc-ms/ms ; saccharomyces-cerevisiae ; magnetic nanoparticles ; methyl esterification ; peptide mixtures ; mouse-liver ; ms analysis
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000259015500031
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/100199]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Houjiang,Ye, Mingliang,Dong, Jing,et al. Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis[J]. journal of proteome research,2008,7(9):3957-3967.
APA	Zhou, Houjiang.,Ye, Mingliang.,Dong, Jing.,Han, Guanghui.,Jiang, Xinning.,...&Zou, Hanfa.(2008).Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis.journal of proteome research,7(9),3957-3967.
MLA	Zhou, Houjiang,et al."Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis".journal of proteome research 7.9(2008):3957-3967.