Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography

	Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography
	Wang, Yanping; Xue, Xingya; Xiao, Yuansheng; Zhang, Feifang; Xu, Qing; Liang, Xinmiao
刊名	journal of separation science
	2008-06-01
卷号	31 期号:10 页码:1669-1676
关键词	flavonoids high efficiency purification preparative parallel HPLC Scutellaria barbata D. Don
通讯作者	梁鑫淼
产权排序	1;1
英文摘要	preparative parallel high performance liquid chromatography combined with solvent partition and other pretreatments were adopted to separate and purify compounds from an extract of scutellaria barbata d. don. mass-triggered fraction collection allowed the rapid and precise isolation of target compounds. twelve compounds were isolated from the extract of s. barbata d. don, their purity in area percent was determined by hplc analysis, and the structures of seven compounds were further identified with hplc/esi-ms, (1)h nmr, and (13)c nmr, among which 4-(3,4-dihydroxy-phenyl)-but-3-en-2-one, acacetin-7-diglucuronide, and luteolin-7-diglucuronide were the first to be identified from this plant. the results demonstrated that multi-channel parallel preparative hplc/uv/ms is an efficient method for isolation and purification of compounds from natural products.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	high-throughput purification ; flavonoids ; information ; libraries ; leaves
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000256987500003
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/100187]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanping,Xue, Xingya,Xiao, Yuansheng,et al. Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography[J]. journal of separation science,2008,31(10):1669-1676.
APA	Wang, Yanping,Xue, Xingya,Xiao, Yuansheng,Zhang, Feifang,Xu, Qing,&Liang, Xinmiao.(2008).Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography.journal of separation science,31(10),1669-1676.
MLA	Wang, Yanping,et al."Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography".journal of separation science 31.10(2008):1669-1676.