Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe

	Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe
	Tian, Ruijun 1,2,3; Li, Lingdong 2,3; Tang, Wei 2,3; Liu, Hongwei 1,2,3; Ye, Mingliang 1,2; Zhao, Zongbao K.2; Zou, Hanfa 1,2
刊名	proteomics
	2008-08-01
卷号	8 期号:15 页码:3094-3104
关键词	2-D nano LC chemical probe interactome isoprenoid chain MS/MS yeast proteome
ISSN号	1615-9853
通讯作者	邹汉法
产权排序	1;1
英文摘要	a chemical proteomic approach was developed for profiling the noncovalent interactome of isoprenoid chain in the yeast proteome. a chemical probe that harbors a biotin moiety and a photoreactive benzophenone group linked to the terminal of geranyl group was synthesized. photoaffinity labeling was performed by incubating the saccharomyces cerevisiae proteome and the probe under 365 nm. uv light. thirty proteins were identified by immobilized neutraavidin enrichment, on-bead digestion, online 2-d nano-lc/ms/ms identification and semi-quantitative proteomic analysis. as noted by gene ontology annotation, the identified proteins demonstrate a wide range of catalytic activity in several biological processes, especially in metabolism and biosynthesis. further data analysis shows that hydrophobic binding of the synthetic probe is potentially the major interaction force leading to covalent labeling. these results argue that intracellular allosteric interactions conferred by the isoprenoid chain of the corresponding chemical structures may be widespread at an interactomic level.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	activated protein-kinase ; virus-rna replication ; fatty-acids ; saccharomyces-cerevisiae ; diphosphate analogs ; mevalonate kinase ; mass-spectrometry ; allosteric sites ; identification ; prenyltransferases
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000258503400011
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/100133]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Tian, Ruijun,Li, Lingdong,Tang, Wei,et al. Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe[J]. proteomics,2008,8(15):3094-3104.
APA	Tian, Ruijun.,Li, Lingdong.,Tang, Wei.,Liu, Hongwei.,Ye, Mingliang.,...&Zou, Hanfa.(2008).Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe.proteomics,8(15),3094-3104.
MLA	Tian, Ruijun,et al."Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe".proteomics 8.15(2008):3094-3104.