AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg

doi:10.1007/s10126-003-0001-0

	AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg
	Li, L; Guo, XM
刊名	MARINE BIOTECHNOLOGY
	2004
卷号	6 期号:1 页码:26-36
关键词	Linkage Mapping Genetic Map Aflp Genome Oyster Crassostrea Gigas
ISSN号	1436-2228
DOI	10.1007/s10126-003-0001-0
文献子类	Article
英文摘要	Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1%) of the markers segregated in a Mendelian ratio, and 94 (26.9%) showed significant (P < 0.05) segregation distortion. Separate genetic linkage maps were constructed for the female and male parents. The female framework map consisted of 119 markers in 11 linkage groups, spanning 1030.7 cM, with an average interval of 9.5 cM per marker. The male map contained 96 markers in 10 linkage groups, covering 758.4 cM, with 8.8 cM per marker. The estimated genome length of the Pacific oyster was 1258 cM for the female and 933 cM for the male, and the observed coverage was 82.0% for the female map and 81.3% for the male map. Most distorted markers were deficient for homozygotes and closely linked to each other on the genetic map, suggesting the presence of major recessive deleterious genes in the Pacific oyster.; Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1%) of the markers segregated in a Mendelian ratio, and 94 (26.9%) showed significant (P < 0.05) segregation distortion. Separate genetic linkage maps were constructed for the female and male parents. The female framework map consisted of 119 markers in 11 linkage groups, spanning 1030.7 cM, with an average interval of 9.5 cM per marker. The male map contained 96 markers in 10 linkage groups, covering 758.4 cM, with 8.8 cM per marker. The estimated genome length of the Pacific oyster was 1258 cM for the female and 933 cM for the male, and the observed coverage was 82.0% for the female map and 81.3% for the male map. Most distorted markers were deficient for homozygotes and closely linked to each other on the genetic map, suggesting the presence of major recessive deleterious genes in the Pacific oyster.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000220334100004
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3565]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, L,Guo, XM. AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg[J]. MARINE BIOTECHNOLOGY,2004,6(1):26-36.
APA	Li, L,&Guo, XM.(2004).AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg.MARINE BIOTECHNOLOGY,6(1),26-36.
MLA	Li, L,et al."AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg".MARINE BIOTECHNOLOGY 6.1(2004):26-36.